顺铂对人宫颈癌Hela细胞超弱发光影响的观察

Effect of DDP treatment on ultraweak luminescence in human cervical carcinoma HeLa line

目的:探讨人宫颈癌Hela细胞株经顺铂(DDP)作用后其超弱发光与细胞增殖活性变化的关系.方法:选择DDP诱导人宫颈癌Hela细胞株,比较人宫颈癌Hela,Hela+DDP细胞株形态变化.MTT比色法、流式细胞仪检测细胞生长周期变化及凋亡情况;用IFFM-D型流动式化学发光仪检测Hela,Hela+DDP细胞的超弱发光强度变化.结果:DDP对Hela细胞有生长抑制作用,并呈时间和浓度依赖性,其48 h的IC50值为3 mg/L,当DDP浓度在3 mg/L以下时,对Hela细胞无明显毒性作用,超过此浓度时,其毒性呈剂量效应关系(P<0.01).流式细胞仪结果表明,与Hela细胞相比较,Hela+DDP细胞G2期细胞数增多,而G1,S期的细胞数明显减少(P<0.01);细胞凋亡率在24,48,72 h分别为(11.4±5.8)%,(21.8±7.9)%,32.5±11.6)%.在1×10-4mol/L鲁米诺及3 mL/L双氧水(H2O2)条件下超弱发光强度随着时间的变化,Hela+DDP细胞明显降低(P<0.01).结论:在DDP非毒性剂量作用后,Hela+DDP细胞超弱发光强度降低,提示超弱发光检测可能作为筛选敏感化疗药物的一项指标.

AIM： To study the changes of human cervical carci- noma Hela line in ultraweak luminescence and the proliferation activation following DDP treatment. METHODS： Hela cells were selected and exposed to different concentrations of DDP. The acti- vation of the cell line, the percentage of apoptosis and the changes of cell cycle were detected by MTT and flow cytometry at different time points. The intensity of uhraweak luminescence was exam- ined by IFFM-D chemil analysis. RESULTS ： DDP inhibited the growth of Hela cell line and the effects were time-and concentration-depended. The IC50 of DDP was 3 mg/L at the 48 h point and DDP had no significant cytotoxicity on Hela cell line when its concentration was less than 3 mg/L. The cytotoxicity in- creased with the enhanced concentration of DDP. After the treatment of DDP, the percentage of apoptosis was （ 11.4 ± 5.8 ） %, （21.8 ±7.9）% and （32.5 ±11.6）% respectively at the 24, 48 and 72 h time points. The number of cells increased in G2 phase and decreased remarkably in G1, S phase（P 〈0.01 ）. The intensities of uhraweak 1 also significantly reduced at different time points （P 〈 0.01 ）. CONCLUSION： The intensi- ties of ultraweak 1 in Hela cell line are reduced after the treatment of DDP at non-toxicity dosage, suggesting that the ultra weak 1 can be used as an indicator in screening sensitive chemotherapeutic agents.