SP和CGRP阳性神经元在大鼠上橄榄复合体中的分布

Distribution of substance P(SP) and calcitonin-gene related peptide (CGRP) positive neurons in rat superior olive complex

目的:研究大鼠上橄榄复合体(SOC)中P物质(SP)和降钙素基因相关肽(CGRP)免疫反应阳性神经元的分布特点,并探讨其意义.方法:采用免疫组织化学及免疫荧光双标记技术,对大鼠脑干进行冰冻切片,在显微镜下观察SP和CGRP免疫阳性神经元胞体及神经纤维的分布特点.结果:SP阳性神经元胞体主要分布在斜方体核(Tz),CGRP阳性神经元胞体主要分布在外侧上橄榄核(LSO)及Tz,在Tz中尚可见大量CGRP阳性神经终末包绕SP和CGRP双标记神经元胞体.结论:CGRP与SP在听觉传导功能中有协同作用,且Tz内SP阳性神经元可能参与了对LSO内CGRP阳性神经元的调控,进而参与调节橄榄耳蜗束的神经活动.

AIM： To study the distribution of neurons which are positive for substance P （SP） and calcitonin-gene related peptide （CGRP） in rat superior olivary complex and to explore its signifi- cance. METHODS ： Using immunohistoehemical and immunoflu- orescent double-staining techniques, We examined the distribution of SP and CGRP positive neurons and fibers on rat ＇s brainstem sections. RESULTS： SP positive neurons were located mainly in trapezoid boby （Tz） , while CGRP positive neurons were located mainly in lateral superior olive （LSO） and Tz. We also found plenty of CGRP positive nerve terminals, which surrounded both SP and CGRP positive neurons. CONCLUSION： SP and CGRP have some synergistic effect in hearing conduction. SP positive neurons in Tz may probably are involved in the regulation of CGRP positive neurons in LSO, which contributes to the regulation of the nerve activities of olivocochlear bundle.