P物质在成骨细胞分化过程中对转录因子Osterix表达的影响

Effect of substance P on expression of Osterix in osteoblast differentiation

目的:在成骨细胞分化过程中检测转录因子Osterix的表达,探讨P物质调控成骨细胞分化及骨形成的分子机制.方法:原代培养大鼠骨髓基质干细胞(rMSCs),并诱导其向成骨细胞定向分化.采用RT-PCR法检测转录因子Osterix在P物质和/或P物质NK1受体拮抗剂L732138干预下的表达.结果:在诱导成骨细胞定向分化的过程中,与空白对照组相比,P物质组转录因子Osterix的表达增强(LSD-t=0.117,P<0.01),P物质NK1受体拮抗剂L732138组转录因子Osterix的表达差异无统计学意义(LSD-t=0.008,P>0.05);与P物质组相比,P物质与P物质NK1受体拮抗剂L732138组转录因子Osterix的表达降低(LSD-t=-0.172,P<0.01).结论:P物质可以促进转录因子Osterix的表达,其受体拮抗剂L732138具有拮抗作用;P物质可通过促进转录因子Osterix的表达,促进成骨细胞分化及骨形成.

AIM： To explore the molecular pathway of substance P-induced osteoblast differentiation. METHODS： Mesenchymal stem cells of rats （rMSCs） were isolated, cultured and induced to differentiate osteoblast. Under the interference of substance P or/ and substance P antagonist L732138, total RNA was extracted and Osterix gene expression was evaluated by RT-PCR. RESULTS： Compared with that in normal control group, the expression of Osterix increased significantly in substance P group（ LSD-t = 0.117, P 〈0.01 ） in the course of osteoblast differentiation, but no sig- nificant difference was seen compared with that in substance P receptor antagonist L732138 group （ LSD-t = 0. 008, P 〉 0.05 ）. Compared with that in substance P group, the 0sterix expression decreased significantly in the federal group of substance P and substance P receptor antagonist L732138 （ LSD-t = - 0. 172, P 〈 0.01 ）. CONCLUSION： Substance P stimulates the expression of Osterix, which can be depressed by substance P receptor antag- onist L732138. Substance P stimulates osteoblast differentiation by stimulating the expression of Osterix.