大鼠胚泡着床障碍动物模型的建立

Establishment of Rats Dysfunctional Embryo Implantatio Model

目的建立大鼠胚泡着床障碍动物模型。方法将与雄性大鼠交配后的成年雌性SD大鼠随机分为正常组、小剂量组(5mg/kg)、中剂量组(6mg/kg)、大剂量组(8mg/kg),小剂量组、中剂量组、大剂量组于妊娠第3天皮下一次性注射不同剂量的米非司酮,于妊娠第8天观察大鼠胚泡着床率及平均着床胚泡数,筛选最佳造模剂量。结果与正常组相比,小剂量组和中剂量组着床率和平均着床胚泡数均显著降低(P<0.01)。中剂量组的着床率和平均着床胚泡数低于小剂量组组,差异无统计学意义(P>0.05)。当剂量达到8mg/kg时,胚泡着床被完全抑制。结论于妊娠第3天皮下注射5mg/kg的米非司酮,可建立稳定可靠的大鼠胚泡着床障碍模型。

Objective To found the model of the dysfunctional embryo implantation rats. Methods Pregnant rats were randomly divided into the control group, the small dose group （5 mg/kg）, the middle dose group （6 mg/kg）and the large dose group（8 mg/kg）. Rats in the latter three groups received different injection dose of mifepristone solution at the third day of pregnancy of pregnant rats, in order to induce the dysfunctional embryo implantation model. Results The pregnancy rates and the average implantation numbers in the small dose group and the middle dose group were greatly reduced compared with the control group（P 〈0.01 ）. There were no obvious differences for the pregnancy rate and average implantation number between the small dose group and the middle dose group, but the average implantation number in the middle dose group was still lower than that of the small dose group. When the injection dose of mifepristone solution reached to 8 mg/kg, the number of rats＇ embryo implantation was none. Conclusion To inject mifepristone solution of 5 mg/kg at the third day of pregnancy of pregnant rats can found a stable model of the dysfunctional embryo implantation rats.