短期联合应用灰黄霉素、克霉唑和地塞米松对红色毛癣菌的影响

Combination antifungal activity of griseofulvin,clotrimazole and dexamethasone

目的研究灰黄霉素、克霉唑及灰黄霉素、克霉唑与地塞米松组合成的联合药物对红色毛癣菌在体内、外活性影响。方法在体外分别应用灰黄霉素、地塞米松、克霉唑、灰黄霉素∶克霉唑∶地塞米松=5∶5∶1联合药物的不同药物浓度作用于红色毛癣菌,确立药物最小抑菌浓度(MIC)和联合药物的分数抑菌浓度(FIC);制作由红色毛癣菌引起皮肤癣的豚鼠动物模型,根据豚鼠用药部位分成四组,对豚鼠皮疹进行疗效的评估。结果体外实验联合药物的MIC小于单药的MIC,且联合药物起协同和次协同作用;体内实验中联合用药组对动物受损局部皮肤给药后,对红色毛癣菌所致皮损积分为(1.89±0.68),与对照组(3.33±0.69)比较,可明显减轻局部皮肤损伤病变程度,该药优于克霉唑组(2.33±0.69)、灰黄霉素组(2.11±0.58)。结论短期内灰黄霉素、克霉唑与地塞米松组合成的联合药物有较强的抗红色毛癣菌和抗炎作用,其抗菌活性强于单独药物的灰黄霉素和克霉唑。

Objective To study combination antifungal activity of griseofulvin, clotrimazole and dexamethasone. Method Different concentration of drugs, such as griseofulvin, clotrimazole, dexamethasone and the combination of griseofulvin, clotrimazole and dexamethasone （griseofulvin/clotrimazole/dexamethasone = 5： 5： 1 ） was used to determine their minimus inhibited concentration （MIC） on the Trichophyton rubrum in vitro , and the Fractional inhibitory concentration （FIC） was calculated. Guinea-pig animal model of dermatophytosis was divided into four groups. The therapeutic effect of guinea pig tinea corporis models was evaluated according to skin lesions and scurf. Results The MIC of concentration of drugs in vitro is smaller than the MIC of single drug,and the concentration of drugs made the hypo-cooperation function;The combination groups are used on guinea pig tinea eorporis models, to the Trichophyton rubrum cause of the skin damage integral are （ 1.89 ± 0.68 ）, contrast with the antitheses groups （ 3.33± 0.69） , it can reduce the degree of skin damage obviously, this drug is better than clotrimazole groups （ 2.33 ± 0.69 ） and griseofulvin groups （ 2.11 ± 0.58 ）. Conclusion The combination of griseofulvin, clotrimazole and dexamethasone show significant antifungal effect, which is better than single drug.