Survivin磷酸化在口腔黏膜下纤维性变及其癌变组织中的表达

Expression of Survivin phosphorylation in carcinogenesis of oral submucous fibrosis

目的检测凋亡抑制蛋白Survivin活性形式——Survivin Thr34位磷酸化(p-Survivin)在口腔黏膜下纤维性变及其癌变组织中的表达,探讨Survivin在OSF癌变过程中的作用及意义。方法应用免疫组化SP法检测10例正常口腔黏膜组织、40例OSF组织及42例OSF癌变组织中p-Survivin的表达,并以Western Blot分析p-Survivin的表达。结果正常口腔黏膜上皮组织中无p-Survivin表达;OSF癌变组p-Survivin阳性表达率(97.6%)高于OSF组(50.0%);而OSF早、中、晚期3组间p-Survivin阳性表达率无显著性差异。Western Blot验证了免疫组化的检测结果。结论p-Survivin在OSF癌变过程中可能起促进作用,为OSF早期诊断和治疗提供理论依据。

Objective To investigate the expression of phosphorylation of Survivin on Thr34 in the tissues of oral submucous fibrosis （OSF） and oral squamous cell carcinoma （OSCC） originated from OSF, and to elucidate an important role of Survivin in the carcinogenesis of OSF. Methods The expression of Survivin Thr34 phosphorylation was examined by SP method in 10 cases of normal oral mu- cosa, 40 cases of OSF and 42 cases of OSCC originated from OSF. Western blot was used to confirm the expression of Survivin Thr34 phospho- rylation in these tissues. Results The expression of Survivin Thr34 phosphorylation was negative in normal oral mucosal epithelium. The positive staining rate of Survivin Thr34 phosphorylation in OSCC originated from OSF （97.6%） was significantly higher than that in OSF （50.0 % ） （ P 〈 0.01 ）. However, the positive staining rate of Survivin Thr34 phosphorylaiton showed no significant difference among the early stage, the moderately advanced stage and the advanced stage of OSF （ P 〉 0.05）. Next, Western blot analysis was used to confirm the phos- phorylation of Survivin on Thr 34 in the carcinogenesis of OSF. Conclusion The expression of Survivin Thr34 phosphorylation in the OSF and OSCC originated from OSF provided the strong evidence that Survivin might play an important role in the carcinogenesis of OSF and served as a new target for diagnosis and treatment.