仙灵骨葆延缓前交叉韧带切断后大鼠关节软骨退行性变的研究

EFFECT OF XIANLINGGUBAO IN SUPPRESSING ARTICULAR CARTILAGE DEGENERATION OF ANTERIOR CRUCIATE LIGAMENT TRANSECTION MODEL IN RATS

目的观察仙灵骨葆(xianlinggubao,XLGB)对前交叉韧带切断术(anterior cruciate ligament transection,ACLT)后骨关节炎大鼠模型膝关节软骨和软骨下骨的影响。方法24只3月龄雌性SD大鼠按体重随机分为3组(n=8):假手术组(A组)、ACLT组(B组)和XLGB组(C组)。B组和C组大鼠行右膝关节ACLT;A组同法打开关节腔暴露ACL后缝合。术后4dC组开始持续XLGB灌胃250mg/(kg·d),A、B组给予等剂量生理盐水。术后12周取材行大体观察,关节软骨Mankin法评分,MMP-13免疫组织化学染色观察关节软骨的退行性变和软骨基质的降解程度,股骨远端软骨下骨骨密度和胫骨近端骨组织形态计量学分析。结果术后12周大体观察A组无溃疡,C组较B组溃疡面小;C组关节软骨Mankin评分和MMP-13免疫组织化学染色积分光密度值低于B组,差异有统计学意义(P<0.05)。B组股骨内侧髁骨密度低于A组和C组,差异有统计学意义(P<0.05);C组的胫骨近端骨量高于B组,差异有统计学意义(P<0.05)。结论XLGB250mg/(kg·d)灌胃12周可以预防ACLT大鼠关节软骨退行性变,其作用机制可能与下调MMP-13的表达和增加软骨下骨骨量有关。

Objective To investigate theffeffects of xianlinggubao （XLGB） on subchondral bone and articular cartilage in the rat osteoarthritis model induced by anterior cruciate ligament transection （ACLT）. Methods Twenty-four 3-month-old female SD rats were divided randomly into 3 groups （n=8）： Sham group （group A）,ACLT group （group B） and XLGB group （group C）. The osteoarthritis model was made by ACLT in groups B and C,the joint cave was sutured after exposure of ACL in group A. After 4 days,XLGB was given at 250 mg/（kg·d） in group C and theffequivalent amount of saline was given in groups A and B. After 12 weeks,the gross appearance of femoral condyles was observed,the degree of cartilage degeneration was scored by Mankin scoring system. The immunostaining for MMP-13 was performed to investigate theffe ect of XLGB on prevention of cartilage matrix loss. The bone mineral density （BMD） measurement and bone histomorphometric analysis were done in subchondral bone of right distal femur and proximal tibia after 12 weeks. Results The gross appearance of femoral condyles showed that ulcer in the group C was smaller than that in group B after 12 weeks. The Mankin’s scale and IA value for MMP-13 in group C were markedly lower than those in group B （P 〈 0.05）. BMD of the subchondral bone in the group B was signi cantly lower than those in the groups A and C （P 〈 0.05）. The bone mass in group C were signi cantly higher than that in group B （P 〈 0.05）. Conclusion Oral administration of XLGB （250 mg/ kg per day） for 12 weeks could prevent the cartilage degeneration of rats after ACLT,down-regulating MMP-13 and increasing subchondral bone mass might participate in this process.