肝癌细胞Survivin的表达及其与化疗药物顺铂敏感性的关系

Relationship between Survivin expression and chemosensitivity of cisplation in liver cancer

目的对临床肝癌标本生存素(Suvivin)基因蛋白的表达进行研究,并分析其与化疗药物敏感性的关系。方法免疫组化SP法检测肝癌标本Survivin表达。对原代培养的肝癌细胞进行顺铂(cisplation,DDP)化学药物处理后检测细胞活性,建立肝癌细胞Survivin表达与化疗药物敏感性的关系。结果Survivin在肝癌中阳性表达率达66.7%(14/21),明显高于癌旁肝组织的9.5%(2/21)。对原代培养的肝癌细胞经过1.0 mg/L DDP处理,24 h后行MTT检测,Survivin阳性组的细胞存活率为47.6%,明显高于Survivin阴性组22.5%,两组间差异有统计学意义(P=0.0014)。结论肝癌细胞高表达Survivin,且Survivin阳性的癌细胞对DDP有一定耐受性,而Survivin阴性的癌细胞对DDP的敏感性较好。Survivin的检测有可能成为预测肝癌化疗敏感性的重要指标。

Objective To investigate the relationship between Survivin gene expression and chemosensitivity of cisplation in liver cancer. Methods Survivin expression was detected with immunohistochemistry in liver cancer. The primary cultured liver cancer cells were treated with cisplation （DDP） and the cellular viability was calculated. Results The survivin over-expressed in liver cancer with the positive rate of 66.7%（14/21） was higher than that in liver tissues with the rate of 9.5%（2/21）. The survival rate of cancer cells in survivin-positive group was 47.6% in average, being higher obviously than 22.5% in survlvin-negative group when the primary cultured cancer cells were treated with 1.0 mg/L DDP, MMT was detected 24 hours later. There was a difference between the survivin-positive group and survivin-negative group （P=0.0014）. Conclusion The liver cancer over-expressed survivin is related to the decrease of cancer cell ehemosensitivity. There is definite tolerance to DDP in cancer cells with positive survivin expression. The detection of survivin may be an important marker to predict the chemosensitivity of liver cancer.