摘要
目的观察一氧化氮(NO)、内皮素(ET)、谷丙转氨酶(ALT)在家兔肝缺血/再灌注损伤过程中的变化及人参多糖(GP)对其的影响。方法30只家兔,随机分为假手术对照组(sham,S)组、肝缺血/再灌注(ischemiareperfusion,IR)组和人参多糖治疗(Ginseng polysauharides,GP)组。分别测定缺血前、缺血45 min及再灌注45min血浆及肝组织NO、ET和ALT含量,电镜观察肝组织形态学改变。结果缺血/再灌注期间,血浆NO明显低于ET及ALT显著高于假手术对照组,尤以再灌注45 min为著;肝组织NO明显低于、ET显著高于假手术对照组;肝组织超微结构发生异常改变。人参多糖可逆转上述指标的异常变化。结论缺血/再灌注导致血管内皮功能紊乱(即NO水平下降和ET水平升高),其在HIRI发生发展中起介导作用;人参多糖可通过提高NO水平和降低ET水平而对肝缺血/再灌注损伤发挥积极的保护作用。
Objective To observe the changes of NO, ET and ALT in rabbit's hepatic ischemia reperfusion injury (HIRI) and the effects caused by Ginseng Polysauharides (GP). Methods Thirty rabbits were randomly divided into 3 groups (n=10, in each): sham operated control group (group S), hepatic ischemia-reperfusion group (group IR) and Ginseng Polysauharides group (group GP). Nitric oxide (NO), endothelin (ET) and alanine aminotransferase (ALT) activities were measured before ischemia, at 45 minutes after ischemia and 45 minutes after reperfusion. Meanwhile NO, ET concentration and ALT activity in liver were measured and the ultrastructive changes of hepar were observed under electron microscope at 45 minutes after reperfusion. Results Compared with group S, NO decreased, ET and ALT increased at 45 minutes and more obvious at 45 minutes after repeffusion in plasma, and NO and ALT decreased but ET increased at 45 minutes after reperfusion in hepar; there were abnormal changes of the hepatic uhrastructure at 45 minutes after reperfusion. GP could reverse the results of indices that is mentioned above markedly. Conclusion The ischemia-reperfu- sion results in dysfunction of the endothelium (NO level decreasing and ET level increasing) which is an important factor during HIRI, GP reduces HIRI by helping balance NO/ET (raising NO level and dropping ET level).
出处
《肝胆胰外科杂志》
CAS
2009年第1期24-26,33,共4页
Journal of Hepatopancreatobiliary Surgery
基金
温州市科技计划资助项目(Y20060195)
关键词
肝脏
再灌注损伤
一氧化氮
内皮素
人参多糖
谷丙转氨酶
兔
liver
reperfusion injury
nitric oxide
endothelin
Ginseng polysauharides
Alanine aminotransferase
rabbit