摘要
目的:探讨caspase-3特异性抑制剂在大鼠缺血再灌注损伤诱导心肌细胞凋亡中的作用。方法:SD大鼠36只,随机分为3组:对照组、I/R3h组和抑制剂组。应用TUNEL法及流式细胞仪分析心肌细胞凋亡的变化,借助caspase-3荧光分析试剂盒,荧光比色法,检测心肌细胞凋亡过程中caspase-3活性的变化。结果:TUNEL及流式细胞仪分析显示caspase-3抑制剂组的AI值和细胞凋亡百分率分别为(4.48±0.98)%,(6.42±1.00)%;与I/R3h组[(18.33%±1.71)%,(29.88±3.74%)]相比明显降低并有显著差异(P<0.01)。caspase-3活性检测显示caspase-3抑制剂组的caspase-3活性明显降低,比I/R3h组降低了45.67%(P<0.01)。结论:caspase-3抑制剂能明显抑制心肌缺血再灌注后的心肌细胞凋亡。caspase-3抑制剂能明显抑制心肌缺血再灌注后的心肌caspase-3蛋白酶活性。
Objective:To investigate the significance of caspase-3 inhibitor in myocyte cell apoptosis induced by myocardial ischemia and reperfusion. Methods:Thirty-six SD rats were divided randomly into three groups, Control group, I/R3h group and caspase-3 inhibitor group. Myocyte cell apoptosis was detected by TUNEL and flow cytometry. The caspase-3 activity was detected by caspase-3 fluorescent assay kit. Results: TUNEL methlod and flow cytometry analysis showed that apoptosis indexes and the percentage of apoptosis cells in caspase-3 inhibitor was (4.48 ± 0.98) % and (6.42% ± 1.00) % respectively, and compared with the I/R3h group [(18.33 ± 1.71) %, (29. 88 ± 3. 74) %] were significantly lower(P〈20.01 ). Caspase-3 activity analysis showed that caspase-3 activity in caspase-3 inhibitor group was reduced 45.67% relative to I/R3h group. Conclusion: Caspase-3 inhibitor can efficiently inhibit myocyte cell apoptosis induced by myocardial ischemia and reperfusion. Caspase-3 inhibitor can efficiently inhibit activity of easpase-3 in cardiomyocytes after ischemia and reperfusion.
出处
《中国临床医学》
2009年第1期12-15,共4页
Chinese Journal of Clinical Medicine
基金
上海市重点学科建设项目(项目编号:B116)