离子跨小鼠肺泡与毛细血管屏障转运的胸膜表面荧光方法

Pleural Surface Fluorescence Measurement of Ion Transport across the Air Space-capillary Barrier

目的:建立一种表面荧光技术测量离子在小鼠离体灌注肺肺泡和毛细血管间的转运。方法:气道内注入含膜不通透性钠离子荧光指示剂Sodium-Green的液体。利用胸膜表面荧光技术测量含有Sodium-Green荧光指示剂的液体注入气道内后,离体灌注肺各种状态下荧光强度所代表钠离子在肺泡内的浓度变化。结果:气道内注入不含钠离子的溶液,灌注液中钠离子浓度由0转换为30mM使Sodium-Green的荧光强度增加(钠离子内流速度:1.2mM·min-1),cAMP激动剂使之增加5倍。AQP1和AQP5缺乏小鼠的钠离子转运无异常。结论:本实验建立了一个间接测量离子在小鼠离体灌注肺肺泡和毛细血管间的转运的胸膜表面荧光方法。

Objective： To develop a pleural surface fluorescence method to measure ion transport in perfused mouse lungs. Methods：The air space of the perfuse lung was filled with aqueous fluid containing membrane-impermeant fluorescent indicators of Na^＋ （Sodium Green）. Record the change of the fluorescence in different condition by pleural surface fluorescence method. Results： After instillation of a Na^＋ -free solution into the air space, an increase in perfusate Na^＋ concentration from 0 to 30 mM gave increased Sodium Green fluorescence （Na^- influx of 1.2mM · min）, which increased approximately fivefold after cAMP agonists. Na^＋ transport was not affected in lungs from mice lacking aquaporins AQP1 or AQPS. Conclusion：Our results establish a pleural surface fluorescence method to measure unidirectional ion flux in intact lung.