三峡库区肺吸虫病与血吸虫感染ELISA检测的交叉反应研究

Analysis of the Cross-Reaction between Paragonimiasis and Schistosomiasis by ELISA in the Three Gorges Reservoir Areas

目的探索三峡库区肺吸虫病与血吸虫病免疫反应的交叉反应率。方法在酶联免疫吸附试验(ELISA)标准条件下进行严格质量控制后,采用ELISA方法分别用肺吸虫成虫抗原检测血吸虫病人阳性血清,用血吸虫虫卵抗原检测肺吸虫病人阳性血清,观察二者间的交叉反应率。结果在质控条件下ELISA的检测结果显示,80例三峡库区肺吸虫患者血清经血吸虫虫卵ELISA检测阳性19例,交叉反应率为23.75%。30例血吸虫患者血清经肺吸虫成虫抗原检测阳性11例,交叉反应率为36.67%。二者间差异无统计学意义(χ2=1.835,P>0.05)。结论三峡库区肺吸虫病与血吸虫病ELISA检测有较高的交叉反应率,需建立肺吸虫和血吸虫病的特异性免疫学检测方法,减少交叉反应。对三峡库区上下游的血吸虫病流行区传入库区的血吸虫病作出特异诊断、早期预防,以达到控制血吸虫病在库区流行、扩散的目的。

Objective To analyze the cross-reaction rate between Paragonimiasis and Schistosomiasis in the three Gorges Reservoir areas. Method Diagnosis by ELISA method was carried out strictly according to quality control measures. Use the soluble egg antigen （SEA） of Schistosomajaponicum to detect the positive serum of Paragonimiasis patiens, also using the adult antigen of Pagumogonimus skrjabini to detect the positive serum of Schistosomiasis patiens analyze the information of the cross-reaction. Result Under the quality control measures, there are 19 positive. Results in the 80 Paragonimiasis patients＇ serum detecting the soluble egg antigen of Schistosomiasis. The cross-reaction rate is 23.75%. And there are 11 positive Results in the 30 Schistosomiasis patiens＇ serum detecting the adult atigen of Paragonimiasis. The cross-reaction rate is 36.67%. No specific difference between the two （χ^2=1.835,P〉0.05）. Conclusion A higher cross-reaction rate exists in Paragonimiasis of the three Gorges Reservoir areas and Schistosomiasis. It is in need of constructing a specific immunologic diagnosis method of both Paragonimiasis and Schistosomiasis in order to diminish the cross-reaction rate. So that it can make a specific diagnosis and promptly treatment of the Schistosomiasis transmitted from up and downstream of the three Gorges Reservoir areas. Also it can make an earlier prevention to control the Schistosomiasis extending in the three Gorges Reservoir areas.