β受体阻滞剂普奈洛尔对大鼠脑创伤后脑组织中IL-1β和IL-1Ra的影响

Influence of the beta-receptor antagonist propranolol on IL-1β and IL-1 Ra after traumatic brain injury in rats

目的探讨β受体阻滞剂(普奈洛尔)对大鼠脑创伤后脑组织中白细胞介素-1β(IL-1β)和白细胞介素-1受体拮抗剂(IL-1Ra)的影响。方法选取SD大鼠72只,随机分为对照组(36只)、治疗组(36只)。采用改良的Feeney法致大鼠右顶叶脑创伤模型,分别给予生理盐水、普奈洛尔腹腔注射(40mg/kg)。每组于致伤后6、24、72小时3个时相点,取右顶叶损伤区脑组织,苏木精-伊红染色(HE染色)法观察其病理变化,免疫组织化学和免疫印迹(W estern b lotting)方法检测其中IL-1β和IL-1Ra的表达。结果对照组:伤后6小时即可见变性坏死神经元,且IL-1β有大量表达,24小时达高峰;IL-1Ra有极少表达并逐渐升高。治疗组:变性坏死神经元明显减少,IL-1β表达降低;而IL-1Ra 6小时即有明显表达并持续至72小时;且IL-1β/IL-1Ra比值较对照组降低。两组比较,差异具有统计学意义(P<0.05)。结论β受体阻滞剂能明显降低大鼠脑损伤区IL-1β表达、提高IL-1Ra表达,可能对减轻大鼠脑创伤后炎性损伤具有一定作用。

Objective To discuss the influence of the beta-receptor antagonist （propranolol） on IL-1β and IL-1Ra in brain traumatic rats. Methods Seventy-two SD rats were randomly divided into two groups： control group （n = 36, treated with normal saline,40mg/kg, ip） and treatment group（ n = 36, treated with propranolol, 40mg/kg,ip）. The cerebral trauma model of rats were made according to the modified Feeney＇s method. The injury areas of right parietal lobe were taken at the moments of 6,24,72 hours after trauma. The pathological change was observed by HE staining. IL-1β and IL-1Ra expressions were measured by the methods of immunohistochemistry and Western blotting. Results Control group ： the denaturation and necrosis of neurons and IL-1β expression were discovered at 6 hours after injury and peaked at 24 hours. While IL-1Ra, progressively increased 6 hours afterwards. Treatment group： The denaturation and necrosis of neurons and IL-123 expression were significantly decreased, but IL-1Ra increased markedly at 6 hours and continued to 72 hours, and the ratio of IL-1β/IL-1Ra reduced. There was statistical significance compared with controls （ P 〈 0.05 ）. Conclusion The beta-receptor antagonist can obviously reduce the expression of IL-1β, increase the expression of IL-1Ra, and exert a protective effect on reducing post -traumatic inflammation lesion in brain traumatic rats.