摘要
用电镜形态计量法检测血小板α颗粒(αG)和致密颗粒(dG)的数密度,用钙荧光指示剂Fura2检测血小板胞质游离Ca2+浓度([Ca2+]i),观察到在钙离子导体A23187作用下,血小板[Ca2+]i明显升高。凝血酶与ADP也都分别引起[Ca2+]i升高,且有浓度依赖性。选用三种激动剂的不同量以反映血小板不同程度激活时,测定[Ca2+]i与颗粒数密度,分析两者间的相关性,发现αG和dG的数密度都与[Ca2+];有紧密的相关性(Pr(αG)<0.05,pr(dG)<0.01),说明血小板的[Ca2+]i升高可促进αG和dG内容的分泌。
The numerical density (Nv) of the α-granule (αG) and dense grantile (dG)measured by using electron dricroscopic morphometry and the cytosolic free calcium concentration ([ Ca2+]i) by using Ca-fluorescent indicator were observed for studying the relationship of [ Ca2+]i and Nv of αG and dG in platelets activated by A23187, thrombin or ADP. The results showed that the [Ca2+]i rose markedly by A23187. Thrombin or ANP could also induce an increase of [Ca2+]i concentration-dependently. Under 3 different degrees of activation induced by different agonists,the close relationship between Nv of both granules, αG and dG, and [Ca2+]i in platelets was found (Pr(αG) < 0.05,Pr(dG) <0.01). These findings indicate that the increase in [Ca2+]i may enhance the secretion of αG and dG.
出处
《生理学报》
CAS
CSCD
北大核心
1998年第2期183-187,共5页
Acta Physiologica Sinica
基金
国家自然科学基金!NO.39370207
关键词
血小板
激活
α果粒
致密颗粒
胞质
钙
,platelet
α-granule
dense granle
cytosolic Ca^(2+)
Fura2