柴胡皂甙-d对DEN致大鼠实验性肝癌形成的影响

the effect of saikosaponin-d on experimental hepatocarcinogenesis Induced by DEN in rats

目的:研究柴胡皂甙d(saikosaponin-d,SSd)对二乙基亚硝胺(diethylnitrosamine,DEN)致大鼠实验性肝癌形成的影响。方法:清洁级雄性SD大鼠90只,随机分为5组:模型组(n=20),对照组(n=10)与SSd大、中、小剂量治疗组(各组n=20)。对照组给予等量生理盐水灌胃,其余各组大鼠均给予2g/L二乙基亚硝胺(DEN,10mg/kg)灌胃,每周5次,同时各治疗组每天腹腔注射不同浓度SSd(2.0,1.5,1.0mg/kg),至16周停药。分别于第6、12、16周处死大鼠,自动生化分析仪测定血清总胆红素(total bilirubin,TBIL)、丙氨酸氨基转移酶(alanine aminotransferase,ALT)、天门冬氨酸氨基转移酶(aspartate amino transferase,AST)和白蛋白(al-bumin,ALB)含量的变化,HE染色观察实验大鼠各期肝组织病理学结构的改变。结果:SSd各治疗组与模型组相比,SSd能够改善大鼠血清各项肝功指标,增加体重,明显减轻大鼠肝细胞的变性坏死,降低死亡率,并且有统计学差异(P<0.05)。病理学检查发现SSd各治疗组大鼠癌结节数及灶的大小均小于模型组,镜下单纯造模组癌细胞呈多形性,异形性明显;相反,SSd各干预组癌细胞分化程度高,异形性较低。结论:SSd对实验性大鼠肝癌的形成有一定的抑制作用。

Objective：To investigate the effect of saikosaponin-d（SSd） on experimental hepatocarcino-genesis induced by diethylinitrosamine（DEN） in rats.Methods：Ninety male SD rats were randomly divided into 5 groups：control group（n=10）,model group（n=20） and three treatment groups（n=20 in each group）.Except for the control group,the other groups were administered（ig） with 2 g/L DEN at a dose of 10 mg/kg body weight（5 times/wk for 16 wk）.At the same time,the rats in the treatment groups were injected intraperitoneally with SSd at different doses（1.0,1.5 and 2.0 mg/kg） once per day for 16 weeks.The rats were anesthetized and killed at 6,12,and 16 week.Hematoxylin and eosin（HE） staining was used to examine the changes of liver pathology and the levels of total bilirubin（TB）,alanine aminotransferase（ALT）,aspartate amino transferase（AST） and albumin（ALB） in serum were measured by biochemical examinations.Results：SSd had activity in protecting rats from toxicity of DEN and can ameliorate liver function,increased the body weight,attenuate the extent of necrosis and reduce the death rate of rat compared with model group（P〈0.05）.Similarly,both the volume and the number of tumors were significantly reduced in 3 SSd treatment groups.Under microscope,the cancer cells in the rats of model group showed polymorphic and abnormal shape.In contrast,the cancer cells in the rats of SSd treatment groups were well differentiated.Conclusion：SSd has inhibitory effect on DEN-induced hepatocarcinogenesis in rats.