白藜芦醇诱导人宫颈癌HeLa细胞凋亡作用的研究(英文)

Study on the resveratrol induced apoptosis in hunman cervical cancer cell line HeLa

目的:探讨白藜芦醇(Res)诱导宫颈癌HeLa细胞凋亡的作用及可能的机制。方法:MTT法检测不同浓度(0、12.5、25、50、100、200、300、400、600μmol/L)Res处理24、48、72h对HeLa细胞的抑制率;流式细胞仪采用AnnexinV和PI双染检测HeLa细胞的凋亡率。荧光显微镜观测细胞的形态学改变。分光光度法检测Caspase-3活性。结果:Res在一定浓度范围内,以浓度和时间依赖的方式抑制宫颈癌HeLa细胞的生长(P<0.05)。以0、200、300μmol/LRes处理细胞48小时,HeLa细胞早期凋亡率分别为2.2%、7.1%、6.23%,晚期凋亡率为7.7%、16.04%、15.43%。荧光显微镜下细胞呈现典型的凋亡性改变。200μmol/LRes处理8h后,Caspase-3活性增加,24h达高峰,后逐渐下降,经50、100、200μmol/LRes处理24h后,Caspase-3活性与对照组相比,分别增加了1.92倍、2.51倍、4.53倍(P<0.05)。结论:白藜芦醇通过诱导Caspase-3活性增加以时间和浓度依赖的方式抑制宫颈癌HeLa细胞增殖,诱导细胞凋亡。

Objective：To explore the effect of resveratrol on the apoptosis of cervical cancer HeLa cell.Methods：HeLa cells were cultured and treated with different concentrations（0,12.5,25,50,100,200,300,400,600μmol/L） of Res for 24,48,72h.The growth inhibition rate was detected by MTT method,and the cell apoptosis was analyzed with flow cytometry（FCM） using annexinV/propidium iodide（PI） double staining.Morphologic configuration of apoptotic cells were observed with fluorescence microscope,and caspase-3 activity was assessed by colorimetric assay.Results：Res inhibited the proliferation of HeLa cells in a time-and dosage-dependent manner（P〈0.05）.After Res treatment at 0,200,300μmol/L for 48h,the apoptosis rates of HeLa cells were 2.2%,7.1%,6.23%,while the necrosis rates were 7.7%,16.04%,15.43%,respectively.The cells showed significant apoptotic morphological changes under fluorescence microscope.After 200μmol/L Res treatment for 8h,the activity of caspase-3 activity increased,and reached the peak at the 24h,then declined gradually.After HeLa cells were treated with 50,100,200μmol/L Res for 24h,the activity of caspase-3 was found increased by 1.92,2.51,4.53 folds compared with that of the control group（P〈0.05）.Conclusion：Res can inhibit the proliferation and induce apoptosis of HeLa cells in a timeand dosage-dependent manner by increasing caspase-3 activity.