乙型肝炎病毒转录体的检测及与隐源性肝病的研究

Serodetection of hepatitis B virus transcription and its relationship with cryptogenic liver disease

目的:探讨不同肝病患者血清中乙型肝炎病毒转录体的表达及与隐匿性感染的关系。方法:用血清核酸提取试剂盒从不同肝病患者血清中提取核酸,PCR及RT-PCR扩增HBVxDNA、fRNA和trRNA,琼脂糖电泳及Southern杂交验证显示结果。结果:HBVxDNA和fRNA在HBsAg(+)血清中检出率显著高于HBsAg(-)者,(P<0.05)。trRNA在HBsAg(+)血清中检出率和HBsAg(-)者无显著差异,(P>0.05)。trRNA在HBsAg(-)的肝癌、肝硬化血清中有高检出率,分别为66.7%、70.0%。此外,在丙肝和其他肝病中trRNA的检出率比HBVxDNA和fRNA的检出率高。结论:HBV病毒转录体与隐匿性感染密切相关。trRNA是某些HBsAg(-)肝病HBV感染的唯一指标,这将为把trRNA作为诊断特殊HBV感染的诊断标志物打下基础。

Objective：To investigate the expression of HBV transcript in sera of different liver patients and its relationship with occult HBV infection.Methods： Nucleic acid was distilled from serum with high pure viral nucleic acid kit.Hepatitis B virus（HBV）X region deoxyribonucleic acid（DNA）,full-length RNA（fRNA）and truncated RNA（trRNA） were amplified by polymerase chain reaction（PCR）and reverse transcriptase-polymerase chain reaction（RT-PCR）.Their products were displayed by agarose electrophoresis and verified by Southern hybridization.Results： HBV xDNA and fRNA were detected higher in HBsAg（＋） serum than that in HBsAg（-） serum,P〈0.05.But there was no significant difference of trRNA in detected HBsAg（＋） serum and HBsAg（-） serum.trRNA had a high rate in HBsAg（-） liver cancer and cirrhosis serum,66.7%,70.0% respectively.trRNA were detected higher than HBVxDNA and fRNA in hepatitis C and other liver disease.Conclusion： HBV transcript shows a close relationship with occult HBV infection.trRNA was the only index of some HBsAg（-） end-stage liver disease,this will lay a foundation for trRNA as the serum marker for the diagnosis of special HBV infection.