人脐静脉内皮细胞的生物学特性及细胞内信号转导机制

The Biological Characteristics of Endothelial Cells of Human Umbilical Vein and the Mechanism of Intracellular Signal Transduction

本实验对人脐静脉内皮细胞（ＨＵＶＥＣ）合成与释放血管活性多肽（ＶＰｓ）及其作用机制进行了研究。结果表明：（１）无神经支配的人脐血管内皮细胞（ＶＥＣ）所含ＶＰｓ较有神经支配的肠系膜血管ＶＥＣ多；（２）这些ＶＰｓ是ＶＥＣ自身合成且能释放到胞外；（３）血管活性肠肽（ＶＩＰ）和Ｐ物质（ＳＰ）使ＨＵＶＥＣ膜上Ｃａ２＋通道开放概率明显增加，生长抑素（ＳＯＭ）使其明显降低，但它们均使胞浆内［Ｃａ２＋］ｉ和ｃＡＭＰ含量明显升高，同时降低ｃＧＭＰ含量。

We observed the synthesis and release of vasoactive polypeptides (VPs) in the endothelial cells of human umbilical blood vessels (HUVEC). The results showed: (1) The content of VPs in the endothelial cells of noninnervated human umbilical vessels is higher than that of mesenteric vessels. (2) These VPs are synthesized by endothelial cells and released into the outside of cell bodies. (3) VIP and SP can stimulate the proliferation of HUVEC, but somatostatin (SOM) inhibits it. (4) VIP and SP increase opening probability of the Ca2+ channel in the membrane of HUVEC, while SOM decrease it, but VIP, SP and SOM result in the increase of concentrations of intracytosolic free Ca2+ and cAMP, while decreasing that of cGMP in HUVEC.In conclusion, we proved that there are local regulatory mechanisms in noninnervated human umbilical blood vessels, and VPs within endothelial cells may play an important role.