大肠癌中聚(腺苷二磷酸核糖)水解酶对聚(腺苷二磷酸核糖)聚合酶的调节作用及意义

Regulatory effects of PARG on PARP in colorectal carcinoma

目的初步探讨大肠癌中聚(腺苷二磷酸核糖)水解酶[Poly(ADP-ribose)glycohydrolase,PARG]与聚(腺苷二磷酸核糖)聚合酶[Poly(ADP-ribose)polymerase,PARP]的关系。方法应用S-P免疫组化检测人大肠癌组织中PARG、PARP的表达;以PARG抑制剂丹宁酸(Gallotannin,GLTN)为处理因素,采用流式细胞计数检测小鼠结肠腺癌CT26细胞中PARG、PARP表达变化。结果PARG和PARP在人大肠癌组织中的表达呈正相关(r=0.300 01,P<0.05);流式细胞计数显示,用GLTN处理后,小鼠结肠腺癌CT26细胞中PARG、PARP的阳性细胞率分别较对照组降低,差异具有统计学意义(P<0.05)。结论大肠癌中PARG和PARP的表达有相关性,PARG抑制剂可以抑制PARG及PARP表达,PARG可能对PARP具有调节作用。

Objective To study the relationship between Poly （ADP-ribose） glycohydrolase （PARG） and Poly（ADP-ribose） polymerase （PARP） in colorectal carcinoma. Methods Immunohistochemical S-P staining was used to examine the expressions of PARG and PARP in 44 specimens human colorectal carcinoma. The positive rate of PARG and PARP in routine colon carcinoma CT26 cells was detected by flow cytometry before and after the treatment of PARG inhibitor Gallotannin. Results PARG and PARP expressed in colorectal carcinoma specimens was of positive correlation （r = 0. 300 01, P 〈 0.05 ）. The expressions of PARG and PARP were weaker in Gallotannin-treated CT26 cells than those in the control cells （P 〈 0.05）. Conclusion The expressions of PARG and PARP are correlated positively in colorectal carcinoma. PARC inhibitor suppresses the expressions of both PARG and PARP, indicating that PARG may regulate PARP.