基因工程杀蚊幼蓝藻稳定性的实验研究

Experimental Study of Stability of Genetically Engineered Larvicidal Cyanobacteria

目的：探讨基因工程藻高效杀蚊幼活性，以确定其质粒表达情况。方法：抗性筛选及蚊虫毒效测试。结果：随机筛选出的基因工程单藻落无一抗性丢失，且３种基因工程藻对淡色库蚊仍具较高毒效，ＬＣ５０（２４小时）Ａ．７１２０ｐＤＣ２６为８．２６×１０３ｃｅｌｓ／ｍｌ；Ａ．ｓｕｂｔｒｏｐｉｃｐＤＣ２６为２．６２×１０４ｃｅｌｓ／ｍｌ；Ａ．ｃｙｌｉｎｄｒｉｃａｔｏｘ为４．２１×１０３ｃｅｌｓ／ｍｌ。结论：穿梭质粒在基因工程藻细胞内稳定遗传，并持续高效表达。

This paper reported the stability and high expression of plasmids of genetically engineered larvicidal Anabaena.The results showed that propagated and cultured in solid medium without antibiotic selection for 3-5 years,the three recombinant Anabaenas kept their resistance to antibiotic and their high larvicidal activity.Their LC 50 (24h) to Culex pipiens pallens were 8.26×10 3 cells/ml (A.7120 pDC26)、2.62×10 4 cells/ml (A.subtropic pDC26) and 4.21×10 3 cells/ml (A.cylindrica tox) respectively.So the genetically engineered Anabaena might be a prospective insecticide for field control on mosquito and the shuttle plasmid might be beneficial to the expression of other genes.