系统性红斑狼疮患者外周血CD4^+CD25^(high)CD127^(low/-)T细胞的表达及其意义

Characteristic of CD4^+CD25^(high)CD127^(low/-)T Cells in Patients with Systemic Lupus Erythematosus

目的检测系统性红斑狼疮患者外周血CD4+CD25highCD127low/-T细胞,探讨调节性T细胞(regulatory Tcells,Treg)在系统性红斑狼疮(systemic lupus erythematosus,SLE)发病中的作用。方法采用三色直接荧光素标记法和多参数流式细胞仪检测41例SLE患者及24名正常对照者外周血CD4+CD25+T细胞、CD4+CD25highT细胞、CD4+CD127low/-T细胞、CD4+CD25+CD127low/-T细胞、CD4+CD25highCD127low/-T细胞的比例,同时检测外周血抗dsDNA等抗体及免疫球蛋白、补体等水平并进行相关分析。结果SLE患者外周血CD4+CD25highCD127low/-T细胞与正常对照组比较明显减少,差异有统计学意义(P<0.001);CD4+CD25highT细胞百分率也低于正常对照组(P<0.001);CD4+CD25+T细胞、CD4+CD127low/-T细胞、CD4+CD25+CD127low/-T细胞百分率与正常对照组比较无显著性差异(P>0.05)。五组细胞与患者年龄、性别和病程,IgG、IgA、IgM、尿蛋白,24h蛋白尿,抗dsDNA、抗Sm、抗SSA、抗SSB、抗RNP、抗C1q、抗核小体抗体水平均无相关性(P>0.05);CD4+CD25+CD127low/-T细胞、CD4+CD25+T细胞、CD4+CD127low/-T细胞与SLEDAI评分无相关性(P>0.05),而CD4+CD25highCD127low/-T细胞及CD4+CD25highT细胞与SLEDAI评分呈负相关(P<0.05)。CD4+CD25highCD127low/-T细胞与CD4+CD25highT细胞呈明显正相关(r=0.987,P<0.001)。结论外周血CD4+CD25highCD127low/-T细胞异常参与了SLE的发病并与SLE活动性相关;CD4+CD25highCD127low/-T细胞可能最具Treg细胞表型特征;CD4+CD25+CD127low/-T细胞可能具有调节和效应双重特性。

To detect Peripheral blood CD4^＋CD25^highCD127^low/-T cells in patients with systemic lupus erythematosus （SLE）. Methods Peripheral blood CD4^＋CD25^＋, CD4^＋CD25^high, CD4^＋CDCD127^low/-T, CD4^＋CD25^highCD127^low/-T and CD4^＋CD25^highCD127^low/-T cells in 41 SLE patients and 24 healthy volunteers were analyzed by 3-color flow cytometry. Levels of anti-dsDNA, immunoglobulin and complement were also measured. Results Peripheral blood CD4^＋CD25^highCD1271^low/-T cells in SLE patients was obviously less than that in control group （P〈0.001）; CD4^＋CD25^higT cells was also less than that of control group （P〈0.001）. In comparison to control group, the level of CD4^＋CD25^＋ CD4＋CD127^low/-, and CD4^＋CD25^highCD127^low/-T cells in patients with SLE didn＇t show a significant change （P〉0.05）. This five group cells had nocorrelation with age, sex, course, IgG, IgA, IgM, urine protein, TPU, anti-dsDNA, anti-Clq, antinuclear body antibody （P〉 0.05）; CD4^＋CD25^highCD127^low/-T ceils, CD4^＋CD25^＋T cells, and CD4^＋CD127^low/-T ceils had no correlation with SLEDAI （P〉 0.05）. But the number of CD4^＋CD25^highCD127^low/-T cells and CD4＋CD25^high T ceils inversely correlated with SLEDAI （P〈 0.001）. CD4^＋CD25^highCD127^low/-Tcells positively correlated with CD4^＋CD25^highT cells （r = 0.987, P 〈 0.001 ）. ConclusionCD4^＋CD25^highCD127^low/-T cells play an important role in the pathogenesis and activity of SEE;CD4^＋CD25^highCD127^low/-Tcells may be the best representative of Treg cells; used as surrogate markers.