摘要
本研究从中药黄芩与止痢灵为质粒消除剂,用携带R质粒的多重耐药性大肠杆菌E.O10株为靶细菌,进行了体外R质粒消除作用的实验研究。同时,观察了黄芩与止痢灵联合应用对R质粒消除的影响。实验结果表明:黄芩和止痢灵对大肠杆菌携带的R质粒具有消除作用。其消除率为2.42%和2.14%。从R质粒的消除表型来看:单用黄芩或止痢灵,绝大多数消除了表现为单一耐药性的丢失。黄芩和止痢灵联合应用,其消除率可提高至18.14%。而且细菌不仅表现为单一耐药性的丢失,还表现对SM+TC,AP+TC多重耐药性的丢失。
The R plasmid curing experinent was performed in vitro with Ecoli 010 strain of bearing R plasmids as target bacteria and Scutellaria baicalensis and zhililing as elimlnation agentsResults showed that when the acting time was 24 hours,the cure rate of R plasmid with Scutellaeia baicalensis and Zhililing was242%_214%The missing patterns of R plasmid might be occurreel in disappearence single resistance.When Scutellaria baicalensis and Zhililing together used.The cure rate of was highness to 1814%and the missing patterns of plasmid might be occurred in disappeaence single and multiple resistance to SM+TC or AP+TC
出处
《中国微生态学杂志》
CAS
CSCD
1998年第2期80-82,共3页
Chinese Journal of Microecology
关键词
大肠杆菌
R质粒
消除
黄芩
止痢灵
Scutellaria baicalensis Zhililing Ecoli Plasmid