微粒体膜苹果酸酶活性与γ-亚麻酸含量的关系

THE RELATIONSHIP BETWEEN THE ACTIVITY OF THE MICROSOMAL MEMBRANE-BOUND MALIC ENZYME AND γ-LINOLENIC ACID CONTENT

用能生产不同GLA含量的油脂的被孢霉为试验材料，研究菌体所产油脂的GLA含量与菌体的微粒体膜结合的苹果酸酶活性之间的关系。结果表明，微粒体膜的苹果酸酶活性与菌体油脂的GLA含量存在显著的正相关关系，由苹果酸酶原位产生的NADPH是微粒体膜磷脂上脂酰基完成会饱和作用的关键所在，胞基质中的NADPH在脂肪酸的延长和去饱和中不能发挥作用。

In this paper, the relationship between γ-linolenic acid (GLA) content of myceliallipid and the achvity of the microsomal membrane-bound malic enzyme of Mortierella isinvestigated. The results indicate that there is a significant positive relativity between themicrosomal malic enzyme activity and the mycelial lipid GLA content. The generation ofNADPH in situ by malic enzyme appeared to be the key of successful desaturation of thefatty-acyl group in the microsomal membrane phospholipids. The NADPH generated in cytosolicmalic enzyme can not act for the desaturation of fatty acid.