摘要
从霉菌和酵母中筛选到一株酵母（Saccharomycescerevisiae231），该菌株具有能够代谢3-脱氧葡糖松（3－deoxyglucosone）的酶，且活性较高。研究了该菌株的最适产酶条件：培养温度28℃，培养基起始pH7.0，培养时间12h，碳源、氮源分别为蔗糖、牛肉膏，添加KH_2PO_4、Ca（H_2PO_4）_2·H_2O能促进产酶。
A yeast strain (saccharomyces cerevisiae 231) was selected from fungi. The stainhas 3-deoxyglucosone-metabolizing enzyme and it's activity is fairly high. The optimumconditions of enzyme production were examined. The optimum temperatUre, initial pH andcultivate bine for enzyme production were 28℃, PH7.0 and 12 hours respectively. The suitablecarbon source of the medium was sucrose and the suitable nitrogen source was beef extractEnzyme activity can increase by adding KH2PO4 or Ca(H2PO4)2. H2O.
出处
《微生物学通报》
CAS
CSCD
北大核心
1998年第2期71-74,共4页
Microbiology China
基金
国家自然科学基金!29466012
广西科学基金
