多孔醋酸纤维素球形载体固定化糖化酶的研究

Study on Porous Cellulose Acetate Beads for Amyloglucosidase Immobilization.

报道了一种多孔醋酸纤维素球形固定化酶载体的制备技术，以ＮａＩＯ４氧化法活化，固定糖化酶，测定了固定化糖化酶的催化反应特性，并与游离酶作了比较．固定化酶在５５℃下水解１０批淀粉溶液，总反应时间超过２４ｈ，活力无显著变化．

Porous cellulose acetate beads are formed for enzyme immobilization. The carrier is activated by being oxidized with NaIO_4, after which amyloglucosidase is attached to it. The optimal reaction conditions and the kinetics of the immobilized amyloglucosidase are determined and compared with those of the free enzyme. The activity of the immobilized amyloglucosidase shows no decay after 10 batches of starch hydrolyses, the total reaction time of which is more than 24 h at 55℃.