环糊精交联固定酶的生物传感器及临床应用

Biosensors and Clinic Application Based on Immobilization of Enzymes with β-Cyclodextrin

通过交联方式将辣根过氧化物酶固定在ＥａｓｔｍａｎＡＱＮ甲基吩嗪修饰电极上，制备成过氧化氢生物传感器．通过循环伏安法和计时电流法证明固定在ＥａｓｔｍａｎＡＱ阳离子交换树脂中的Ｎ甲基吩嗪有效地在辣根过氧化物酶和玻碳电极之间传递电子．由于该生物传感器对过氧化氢具有良好的生物电催化还原的功能，所以将它与葡萄糖氧化酶和半乳糖苷酶结合，制备成双酶和三酶体系的生物传感器，用于葡萄糖和乳糖的测定．该生物传感器具有灵敏度高、响应快、响应范围宽及选择性好等优点．

Biosensor highly sensitive to hydrogen peroxide has constructed by immobilizing horseradish peroxidase in EastmanAQN-methyl phenazine methosulphate modified electrode via crosslinking. Cyclic voltammetry and chronamperometry were employed to demonstrate the effective electron transfer between immobilized horseradish peroxidase and a glassy carbon electrode via N-methyl phenazine methosulphate in EastmanAQ polymer film. Because of high efficiency of bioelectrocatalytic reduction of hydrogen peroxide via N-methyl phenazine methosulphate, the hydrogen peroxide sensor was combined with glucose oxidase and βgalactosidase for bienzyme and trienzymebased biosensor for determination of low glucose and lactose. The biosensors for hydrogen peroxide, glucose and lactose possessed a wide variety of advantages including long stability, rapid response times, wide dynamic range, high sensitivity and selectivity. Comparison of glucose biosensor with colorimetric method with glucose oxidase and peroxidase for the determination of serum glucose from diabetic patients indicates that the results display a good consistency.