摘要
介绍一种噬菌体ＤＮＡ的快速抽提方法．用聚乙二醇沉淀噬菌体颗粒，然后经ＤＥＡＥ纤维素纯化处理和酚抽提．与传统的噬菌体ＤＮＡ纯化方法相比，改进后的方法方便、快速、经济，可获得高纯度的噬菌体ＤＮＡ．
A purification method for phage DNA is introduced. Phage particles are precipitated by polyethylene glycol (PEG), then purified by DEAEcellulose DE52 and extracted by phenol. This modified method is more convenient, rapid and economical than the traditional method of phage DNA purification and can obtain phage DNA with high purity.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1998年第2期186-187,共2页
Progress In Biochemistry and Biophysics
关键词
噬菌体
DNA
抽提
聚乙二醇
phage DNA, extraction, polyethylene glycol (PEG), DEAEcellulose DE52
