利用固定化简单节杆菌转化醋酸可的松

Transformation of Cortisone Acetate Using Immobilized Arthrobacter simplex Cell

用聚乙烯醇海藻酸钙复合载体包埋简单节杆菌ＢＹ２３５１的方法，制备了具有较高机械强度和较好活性的球形固定化细胞。确定了固定化条件、活化条件和酶的特性。在摇瓶中利用该固定化细胞进行醋酸可的松脱氢生成醋酸强的松的反应，初始底物浓度为２０ｇ／Ｌ、反应１８ｈ的转化率可达９８％。在分批次反应中，适时地活化可保持固定化细胞的活性。

A new cell immobilization method is developed in which cells of Arthrobacter simplex BY2351 are immobilized by entrapment with polyvinyl alcoholalginate complex carrier.The immobilized cells preparation contains spherical beads with high mechanical strength and △1dehydrogenase activity.The activation conditions and enzymatic properties of the immobilized cells are studied.When the initial substrate concentration is 20mg/ml,the conversion of cortisone acetate to prednisone acetate by immobilized cells in shake flasks is 98% at 18h.The stability of immobilized cells is maintained by activation at proper time.