摘要
用聚乙烯醇海藻酸钙复合载体包埋简单节杆菌BY2351的方法,制备了具有较高机械强度和较好活性的球形固定化细胞。确定了固定化条件、活化条件和酶的特性。在摇瓶中利用该固定化细胞进行醋酸可的松脱氢生成醋酸强的松的反应,初始底物浓度为20g/L、反应18h的转化率可达98%。在分批次反应中,适时地活化可保持固定化细胞的活性。
A new cell immobilization method is developed in which cells of Arthrobacter simplex BY2351 are immobilized by entrapment with polyvinyl alcoholalginate complex carrier.The immobilized cells preparation contains spherical beads with high mechanical strength and △1dehydrogenase activity.The activation conditions and enzymatic properties of the immobilized cells are studied.When the initial substrate concentration is 20mg/ml,the conversion of cortisone acetate to prednisone acetate by immobilized cells in shake flasks is 98% at 18h.The stability of immobilized cells is maintained by activation at proper time.
出处
《生物工程学报》
CAS
CSCD
北大核心
1998年第2期176-180,共5页
Chinese Journal of Biotechnology
基金
天津市自然科学基金
关键词
醋酸可的松
简单节杆菌
固定化细胞
Cortisone acetate,Arthrobacter simplex,immobilized cells,polyvinyl alcohol