南极冰藻中谷胱甘肽及其相关酶的初步研究

Primary study on glutathione and glutathione-related enzymes of Antarctic ice microalgae

目的研究和发现极端环境下南极冰藻中谷胱甘肽含量及其相关酶活性。方法用分光光度法对24种已鉴定或初步鉴定的南极冰藻的胞内谷胱甘肽含量、谷胱甘肽合成能力(GPA)、谷胱甘肽还原酶活力进行测定。结果南极蓝藻B-1中谷胱甘肽含量最高;南极衣藻ICE-L和南极硅藻GJ01的谷胱甘肽总产量居前2位;南极冰藻的谷胱甘肽合成能力普遍高于常温绿藻,仅2种冰藻谷胱甘肽合成能力低于三角褐指藻。南极硅藻GJ01和南极衣藻ICE-L谷胱甘肽还原酶活力高于对照组。结论南极冰藻成为谷胱甘肽的新来源是有可能的。

Objective To study on glutathione and glutathione-related enzymes of Antarctic ice microalgae. Methods Glutathione （GSH） content, glutathione produce ability （GPA） and glutathione reductase （GR） activity in 24 kinds of Antarctic ice microalgae were assessed u- sing the spectrophotometer methods. Results GSH content of Cyanophyceae B-1 was highest. Total GSH yields of Antarctic ice microalgae Chlamydomonas sp. ICE-L and Berkeleya rutilans GJ01 were in the top two positions. GPA in most Antarctic ice microalgae was higher than that in mesophilic green algae, and only in two ice microalgae was lower than that in Phaeodactylum tricornuturn. GR activities of Antarctic ice microalgae Berkeleya rutilans GJ01 and Chlamydomonas sp. ICE-L were greater than that of the control microalgae. Conclusion It is possible that Antarctic ice microalgae will become a new resource of GSH.