添加羧甲基壳聚糖的藻酸盐印模材料抗菌性能研究

Antibacterial activities of dental alginate impression materials impregnated with carboxymethyl chitosan

目的对添加羧甲基壳聚糖的藻酸盐印模材料的抗菌性进行测试,为口腔抗菌印模材的研究提供参考。方法分别以不同的添加比将不同取代位置、取代度、相对分子质量和脱乙酰度的羧甲基壳聚糖加入藻酸盐印模材料中,采用薄膜密着法,分别测试添加抗菌成分后印模材料对大肠杆菌、金黄色葡萄球菌的抗菌活性。结果O-羧甲基壳聚糖的抗菌性较好,O-羧甲基壳聚糖分别以1%和1.4%的添加比添加到藻酸盐印模材料中,对大肠杆菌和金黄色葡萄球菌有最好的抗菌性。随着脱乙酰度的提高,抑菌率均可达到99.99%。结论添加羧甲基壳聚糖的藻酸盐印模材料具有良好的抗菌效果。

Objective To determine the antibacterial activities of the alginate impression materials impregnated with carboxymethyl chitosan. Methods Carboxymethyl chitosan with different replace positions, replace degrees, relative molecular masses and deacetyl degrees were added into the dental alginate impression materials by different ratios. Their antibacterial activities were determined using a method according to the Chinese and Japanese standards for testing antibacterial efficacy of antibacterial materials. Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC6538 were used in this test. Results O-carboxymethyt chitosan showed best antibacterial activity. O-carboxymethyl chitosan added into the alginate impression materials had the best antimicrobial activity on Escherichia coli and Staphylococcus aureus in the ratios of 1% and 1. 4%, respectively. Its inhibitory bacteria rate could reach 99.99 % along with the increase of deacetyl degree. Conclusion The alginate impression materials impregnated with carboxymethyl chitosan have a favorable antimicrobial activity.