酶解-流动分析法测定烟草中果胶前处理方法的改进

Improvement on Preteatment for Determining Pectin in Tobacco with Enzymolysis-flowanalysis

为提高烟草中果胶的分析速度,对酶解流动分析法中烟叶的前处理过程进行了改进,即用5%醋酸溶液萃取代替80%乙醇溶液回流抽提除去烟末中的水溶性糖,并采用两种方法同时测定了20个烤烟烟叶样品中的果胶含量。结果表明：①改进法果胶的回收率为99.5%～105.7%,变异系数2.08%;②改进法的检测结果稍高于原方法,但二者没有显著性差异。

The sample pretreatment in determining pectin in tobacco by enzymolysis-flowanalysis method was improved in order to accelerate the analytical process.The sample was directly extracted with 5% acetic acid instead of 80% ethanol reflux extraction to remove soluble sugar from tobacco,then analyzed by enzymolysis-flowanalysis.The pectin contents in 20 flue-cured tobacco leaf samples extracted with 5% acetic acid and 80% ethanol respectively were determined.The results indicated that the average recovery of pectin by acetic acid extraction was from 99.5% to 105.7% with the CV of 2.08%.The pectin content determined with acetic acid treatment was slightly higher than that with 80% ethanol,however,the difference was not significant.