女性高血压与口服避孕药及ACE基因关系

Association of oral contraceptives and ACE gene polymorphisms on risk of hypertension in Chinese women:a case-control study

目的评价口服避孕药（OC）的使用和血管紧张素转换酶基因（ACE）多态性联合作用对妇女高血压的发病风险。方法采用病例对照研究的方法,选择665例女性高血压病例和665名女性高血压对照,按照年龄（±3岁）和地区进行匹配。采用限制性片断长度多态性-多聚酶链式反应（RFLP-PCR）方法进行基因型检测。结果（1）ACE基因I/D位点和A2350G位点基因型在病例组和对照组间分布差异无统计学意义;（2）与携带ACE基因ID＋DD基因型且未服用OC的妇女比较,携带ACE基因ID＋DD基因型的妇女服用OC≥15年明显增高患高血压的风险0.66倍（OR=1.66,95%CI=1.11-2.48）;与携带ACE基因AG＋GG基因型且未服用OC的妇女比较,携带ACE基因AG＋GG基因型的妇女服用OC≥15年也明显增高患高血压的风险0.66倍（OR=1.66,95%CI=1.09-2.54）。结论携带ACE基因D等位基因或者G等位基因的妇女服用OC≥15年可以明显增加患高血压的风险。

Objective To evaluate the association of oral contraceptives （OC） use and angiotensin（ACE） gene polymorphisms on the risk of hypertension in Chinese women. Methods We included 665 female hypertensive cases and 665 female controls free from hypertension. All controls were matched with cases on age （ ± 3 years） and residence. The restricted fragment length polymorphism-polymerase chain reaction （RFLP-PCR） was used in genotype assay. Results The genotype distribution of I/D and A2350G polymorphisms were similar between hypertensive cases and controls. Compared with non- OC users with ID ＋ DD genotypes, women carried ID ＋ DD genotypes with OC use for at least 15 years had a significantly increased risk of hypertension（ OR = 1.66, 95 % CI = 1.11 - 2.48 ）. Meanwhile, compared with non - OC users with AG ＋ GGgenotypes, women carried AG ＋ GG genotypes with OC use for at least 15 years had a significantly increased risk of hypertension （ OR = 1.66, 95 % C1 = 1.09 - 2.54）. Conclusion D allele of I/D locus and G allele of A2350G locus were risk alleles of developing hypertension for long term OC users.