丹酚酸对四氯化碳致大鼠肝损伤保护作用

Protective effects and mechanism of salvianolic acid on acute hepatic injury induced by CCl4 in rats

目的探讨具有特定组成的丹酚酸（SA）对四氯化碳（CC l4）所致大鼠肝损伤的预防性保护作用及其机制。方法将健康SD成年大鼠随机分为5组,对照组和模型组给予蒸馏水,各实验组分别用3种不同剂量SA灌胃,2周后以花生油（对照组）或CC l4（其余各组）腹腔注射,24 h后测定大鼠血清天门冬氨酸氨基转移酶（AST）及丙氨酸氨基转移酶（ALT）、血清和肝组织丙二醛（MDA）、谷胱甘肽（GSH）含量以及超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）活性,并观察肝组织病理变化。结果模型组大鼠血清AST、ALT值分别为615 U/L和243 U/L,而SA组大鼠AST、ALT分别为348-452和134-181 U/L,活性显著下降（P〈0.05）。与模型组比较,当SA剂量为300mg/（kg.bw）时,大鼠血清和肝匀浆中的SOD活性分别上升22.7%和16.7%,MDA含量降低19.3%和30.5%,GSH含量升高9.2%和9.1%,差异均有统计学意义（P〈0.05）。实验组和对照组间GSH-Px活性没有显著变化（P〉0.05）。模型组大鼠肝脏小叶中心部出现明显点状和灶状坏死,而SA不同剂量组大鼠肝脏病变明显减轻。结论前期给予适量的SA处理可有效抵抗CC l4所致大鼠急性肝损伤,其保护作用机制可能与提高大鼠机体抗氧化能力有关。

Objective To observe the protective effects of salvianolic acid（SA） in specific composition on CCl4-induced acute hepatic injury of rats and the possible mechanisms. Methods Rats were divided into 5 groups. Water was given to control and model group and SA in different doses to test groups by gavage, respectively. After 2 weeks, acute hepatic injury model was induced by CC14 and oil was used in control group. The activity of aspartate aminotransferase （AST）, alanine aminotransferase （ALT） in serum and the levels of malondi-aldehyde （MDA） , superoxide dismutase （ SOD）, glutathi- one peroxidase （GSH-Px）, and glutathione （GSH） in serum and liver were measured. Pathological changes of liver were also observed. Results The acdvity of AST and ALT in serum of model rats were 615 1U/L,243 IU/L, and 348 -452 IU/ L, 134 - 181 IU/L in SA groups, respectively, with significant decrease （P 〈 0.05 ）. Compared with the model group, the activities of SOD in 300 mg/kg · bw SA group showed 22.7% and 16. 7% increase in serum and livelr homogenate tissue, and the level of MDA a decrease of 19. 3% and 30. 5 %, and GSH an increase of 9. 2% and 9. 1% （ P 〈 0.05 ）. Degeneration and necrosis of liver tissues were observed. Conclusion SA shows protective effect against CC14-induced acute liver injuries in rats, and the mechanism is relevant to the improving of the anti-oxidative activity.