稳恒磁场对新生小鼠肾上皮细胞影响

Effect of static magnetic field on proliferation and differentiation of rat kidney epithelial cells in vitro

目的研究稳恒磁场(SMF)对新生小鼠肾上皮细胞增殖分化的影响。方法利用大鼠新生小鼠肾上皮细胞进行原代培养,观察不同磁感应强度的稳恒磁场(5,10,20,30,40,50,60 mT)作用下,其细胞形态学变化,从细胞计数角度观察细胞生长与分化过程,同时测定蛋白质含量、丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性,并与对照组进行比较。结果经稳恒磁场作用的新生小鼠肾上皮细胞集落形成率明显减少,细胞体积小;磁感应强度为40,50,60 mT各组与对照组比较,其相对蛋白质含量明显降低(P<0.05),MDA的含量明显增高(P<0.01),SOD的活性明显降低(P<0.05),并呈强度-效应关系。结论稳恒磁场能抑制新生小鼠肾上皮细胞增殖和分化,可能与其能抑制蛋白质合成,导致脂质过氧化有关。

Objective To study the effect of static magnetic field （SMF） on differentiation of kidney epithelial cells in rat. Methods The primary culture of new born rat kidney epithelial cells was exposed to SMF at the intensity of 5,10,20, 30,40,50 and 60 mT. The cell culture was observed with methods of cytomorghology and cell counting. And the content of protein, the MTT, malondialdehyde （MDA） and activity of superoxide dismutase （SOD） were determinded. Results The SMF inhibited differentiation of the rat kidney epithelial ceils and affected cell proliferation. In the experimental groups with 40,50,60 mT the relative content of protein decreased and the level of MDA and the activity of SOD decreased obviously （ P 〈 0. 05 ）. Conclusion The inhibition effect of SMF on rat kidney epithelial cells might be associated with protein synthetis and lipid peroxidation.