高效液相色谱法测定人工虫草菌丝中腺苷和虫草素的含量

Determination of Adenosine and 3'Deoxyadenosine in Cordyceps militaris (L.) Link.by HPLC

为定量测定蛹虫草菌丝体中腺苷和虫草素的含量，用ＹＷＧＣ１８不锈钢柱，磷酸二氢钠氢氧化钠缓冲液（ｐＨ６８，加１％四氢呋喃）为流动相，高效液相色谱法同时分离和测定了人工蛹虫草菌丝中腺苷和虫草素，并考察了提取方法。二组分的线性关系良好。

The establishment of a quantitative determination method by HPLC for adenosine and 3'deoxyadenosine in Cordyceps militaris is reported.The sample was extracted with water under ultrasonic management for 2 hours.Chromatographic conditions:YWG C18 10μm 5mm×200mm;mobile phase consisted of NaH2PO4NaOH buffer solution (pH 6.8);UV detection at 260nm.The recoveries of adenosine and 3'deoxyadenosine were 95.8%±0.64% and 100.6%±0.65%(n=5) respectively.