大鼠原位辅助性肝移植治疗急性肝功能衰竭

The treatment of acute liver failure by auxiliary partial orthotopic liver transplantation in rats

为了评价大鼠原位辅助性部分肝移植（ＡＰＯＬＴ）对急性肝功能衰竭的支持作用。切除７５％的肝脏并阻断残余肝脏的血供５０分钟诱导大鼠急性肝功能衰竭。治疗组受体肝脏切除７５％并将３０％的供肝植于原位，然后阻断残余的右上叶和右下叶之血供５０分钟。结果显示，大鼠急性肝功能衰竭的５天生存率仅３３％，而接受ＡＰＯＬＴ者５天生存率和移植肝存活率分别为８０％和７３％，术后第５天肝功能基本恢复正常。可见，大肝切除和余肝缺血诱导的大鼠急性肝功能衰竭是一个理想的动物模型。

The models of both auxiliary partial orthotopic liver transplantation (APOLT) and acute liver failure were established. Acute liver failure in rats was successfully induced by partial hepatectomy of 75% with the remnant liver ischemia for 50 min. In treated group, the recipients underwent APOLT before the remnant liver (the right superior and inferior lobes) receiving temporary ischemia for 50 min. The experimental results showed that the 5day survival rate of the rats with acute liver failure was only 3%, while in the rats receiving APOLT, the 5day survival rate of the recipients and the liver graft was 80% and 73%, respectively. The liver function returned near to normal level on the 5th postoperative day. It is suggested that acute liver failure induced by major liver resection with remnant liver ischemia in rat is an ideal model and APOLT might provide an exact support for experimental acute liver failure in rat.