大黄素对人胆管癌细胞株QBC_(939)的抑制作用

Study on the growth-inhibited effect of Emodin on the human cholangiocarcinoma cell line QBC_(939)

目的探讨中药成分大黄素（EM）联合化疗药物阿霉素（ADM）对人胆管癌细胞株QBC939的抑制作用及其机制。方法药物对肿瘤细胞抑制率检测采用四甲基偶氮唑蓝（MTT）比色法；多药耐药P糖蛋白（P-gp）以免疫组织化学法检测。结果单用ADM培养时IC50为36．60μg／mL，细胞抑制率分别为37．96％～94．87％，加用不同浓度EM培养后IC50分别为29．68、24．78、17．22和17．18μg／mL，两两比较，P均〈0．05，且均显著低于单用ADM者（P均〈0．05）；细胞抑制率均显著低于单用ADM者，且抑制能力呈浓度依赖性。结论EM可增加化疗药ADM对胆管癌呱39细胞株的抑制作用，可明显下调P—gP的表达，增强胆管癌细胞对化疗药的敏感性。

Objective To investigate the growth-inhibited effect of emodin on the human cholangiocarcinoma cell line QBC939 and to illustrate its initial mechanism. Methods Using tetrazolium dye assay, effects of various concentrations of EM on sensitivity to the human cholangiocarcinoma cell line QBC939 was studied. And P-glycoprotein was measured by immunohistochemistry. Results IC50 was 36.60μg/mL and the inhibitive rate was 37.96 96 -94.87 % respectively when the human cholangiocarcinoma cell line QBC939 were cultured with adriamycin only. However when different concentration of emodin was added, IC50 was 29.68 μg/mL, 24.78μg/mL, 17.22μg/mL and 17.18μg/mL, the differences among 4 groups had statistical significance （P 〈 0.05）. The inhibitive rates in different concentration emodin groups were all lower than those in adriamycin only groups. And under the same concentration of adriamycin, the degree of reverse was dependent on the concentration of emodin. Conclusion EM was able to enhance the relative sensitivity of chemotherapeutics to the human cholangiocarcinoma cell.