p53和bcl-2表达与BT325细胞辐射性凋亡的关系

Relationship between oncogene expression and radiation induced apoptosis of BT325 cell line.

目的探讨癌基因表达的变化与辐射诱导肿瘤细胞凋亡的关系。方法采用免疫组化技术检测人多形性胶质母细胞瘤细胞系，经Ｘ射线处理前后ｐ５３、ｂｃｌ－２基因表达的变化；原位杂交方法检测ｐ５３基因在ｍＲＮＡ水平表达的变化。结果①凋亡组内ｐ５３蛋白表达率明显高于对照组，ｂｃｌ－２表达显著减弱，ｐ５３与ｂｃｌ－２蛋白表达呈显著性负相关。②凋亡组内ｐ５３在ｍＲＮＡ水平表达量明显高于对照组，ｐ５３基因在蛋白水平与ｍＲＮＡ水平表达呈显著性正相关。

Objective\ To investigate the relationship between mechanism of radiation induced apoptosis and the expresion of p53,bcl 2 genes.Methods\ Immunohistological staining assay (ABC assay) was used to detect the changes of p53,bcl 2 after radiation.p53 mRNA was examined by in situ hybridization in BT325 cell line after low dose radiation.Results\ ①Overexpression of p53 was detected in apoptotic groups (vs.control: P <0 01).Expression of bcl 2 decreased in the apoptotic group ( P <0 01).The labeling index (LI) of p53 gene was significantly negatively correlated with the LI of bcl 2(0 01< P <0 05),②The LI of p53 mRNA in apoptotic groups was significantly higher than that in the control ( P <0 01).Oncoprotein of p53 gene was significantly positively correlated with transcription of p53 mRNA( P <0 01).Conclusion\ p53 gene has promotive or inhibitory effect on radiation induced apoptosis.bcl 2 gene is an antagonist to apotosis.p53 gene and bcl 2 gene have synergetic or antagonistic relationship in the process of apoptosis.