电镜形态和DNA链断裂研究^(153)Sm内照射诱发骨肉瘤细胞凋亡

Electron microscopic morphology and DNA chain fragmentation studies on apoptosis in bone tumor cells induced by 153 Sm.

目的对１５３Ｓｍ－ＥＤＴＭＰ内照射诱发骨肉瘤细胞凋亡和ＤＮＡ链断裂程度进行了研究。方法观察１５３Ｓｍ－ＥＤＴＭＰ内照射不同时间，诱发骨肉瘤细胞凋亡的电镜形态及ＤＮＡ链断裂程度。结果研究发现，在１５３Ｓｍ－ＥＤＴＭＰ内照射作用下，可呈现核断裂，核染质边聚，以及膜包裹着的凋亡小体形成的骨肉瘤细胞凋亡的形态特征。并且观察到随着１５３Ｓｍ－ＥＤＴＭＰ内照射作用时间的延长，则骨肉瘤细胞的ＤＮＡ链断裂程度亦随之增升。结论１５３Ｓｍ－ＥＤＴＭＰ内照射治疗时，可诱发骨肉瘤细胞凋亡发生。

Objective\ To study the apoptosis and fraction of DNA chain fragmentation in bone tumor cells induced by 153 Sm EDTMP.Methods\ Apoptosis in bone tumor cells exposed to different time periods of 153 Sm EDTMP internal irradiation was observed by electron microscopic morphology and DNA chain fragmentation studies.Results\ It was demonstrated that the bone tumor cells internally exposed to 153 Sm EDTMP displayed nuclear fragmentation,margination of condensed chromatin,and formation of membrane bounded apoptotic bodies.Our study showed that the percentage of DNA chain fragmentation increases in direct proportion to the duration of internal exposure to 153 Sm EDTMP.Conclusion\ Apoptosis induced by 153 Sm EDTMP in bone tumor cells was dependent on the time of 153 Sm EDTMP exposure.