摘要
目的对153Sm-EDTMP内照射诱发骨肉瘤细胞凋亡和DNA链断裂程度进行了研究。方法观察153Sm-EDTMP内照射不同时间,诱发骨肉瘤细胞凋亡的电镜形态及DNA链断裂程度。结果研究发现,在153Sm-EDTMP内照射作用下,可呈现核断裂,核染质边聚,以及膜包裹着的凋亡小体形成的骨肉瘤细胞凋亡的形态特征。并且观察到随着153Sm-EDTMP内照射作用时间的延长,则骨肉瘤细胞的DNA链断裂程度亦随之增升。结论153Sm-EDTMP内照射治疗时,可诱发骨肉瘤细胞凋亡发生。
Objective\ To study the apoptosis and fraction of DNA chain fragmentation in bone tumor cells induced by 153 Sm EDTMP.Methods\ Apoptosis in bone tumor cells exposed to different time periods of 153 Sm EDTMP internal irradiation was observed by electron microscopic morphology and DNA chain fragmentation studies.Results\ It was demonstrated that the bone tumor cells internally exposed to 153 Sm EDTMP displayed nuclear fragmentation,margination of condensed chromatin,and formation of membrane bounded apoptotic bodies.Our study showed that the percentage of DNA chain fragmentation increases in direct proportion to the duration of internal exposure to 153 Sm EDTMP.Conclusion\ Apoptosis induced by 153 Sm EDTMP in bone tumor cells was dependent on the time of 153 Sm EDTMP exposure.
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
1998年第2期89-91,共3页
Chinese Journal of Radiological Medicine and Protection
基金
国际原子能机构资助
关键词
电镜
DNA链断裂
钐153
骨肉瘤
细胞凋亡
Electron microscopy\ Morphology\ DNA chain fragmentation\ 153 Sm EDTMP\ Internal irradiation\ Bone tumor cells\ Apoptosis