系统性红斑狼疮病人T和B淋巴细胞凋亡的观察

Accelerated Apoptosis of T and B Lymphocytes from Patients with Systemic Lupus Erythematosus

为了研究SLE的T、B淋巴细胞及其亚类的凋亡情况,采用碘化丙锭染色,在流式细胞仪下观察计数:22例SLE病人淋巴细胞(包括总体淋巴细胞、CD3^+、CD4^+、CD8^+T细胞和CDl9^+B细胞)凋亡率在培养0、24、48h时均较正常组有显著增高,并以CD4^+T细胞和CDl9^+B细胞在活动期SLE病人中凋亡更突出。此外,SLE病人,自身抗体产生愈多,其细胞凋亡率愈高;疾病活动度增高,凋亡率也较高。提示;SLE病人的淋巴细胞凋亡在体外加速,与其自身抗体产生有密切关系,在其发病机制中起一定的作用。

Using flow cytometry and propidium iodide (PI) staining, the rates of apoptosis in different lymphocyte subsets from patients with systemic lupus erythematosus (SLE) were investigated. The rates of lymphocyte apoptosis (including total lymphocytes, CD3+, CD4+, CDS+T cells and CD19+ B cells) from 22 patients with SLE were significantly increased at tO (P<0. 05)、 t24 (P<0. 005) and t48 (P<0. 001) compared with 16 normal controls. In active SLE patients, CD4+ T lymphocytes and CD19+B lymphocytes showed higher levels of apoptosis. The higher the rates of apoptosis, the more the production of autoantibodies, and so did between the degree of disease activity and the rates of apoptosis. The results indicate that apoptosis of lymphocytes in SLE accelerate in vitro, by which autoantibodies were produced. The abnormalities of lymphocyte apoptosis may contribute to the pathogenesis of SLE.