氯胺酮对大鼠脑NO/cGMP信号转导系统的影响

Effects of ketamine on nitric oxide/cyclic guanosine 3',5'-monophosphate signaling pathway in the rat brain in vivo

目的:了解氯胺酮对大鼠不同脑区一氧化氮合酶(NOS)活性,一氧化氮(NO)产量和环鸟苷酸(cGMP)含量的影响。方法:SD大鼠32只,随机分为对照组和氯胺酮组,分别腹腔注射(ip)生理盐水10ml·kg^(-1)和氯胺酮100mg·kg^(-1),用分光光度法测定NOS活性和NO产量,放射免疫法测定cGMP含量。结果:大鼠氯胺酮100mg·kg^(-1)ip能明显抑制小脑、大脑皮层、海马的NOS活性(P<0.05或P<0.01),并明显减少上述脑区的NO产量和cGMP含量(P<0.05或P<0.01)。结论:NO/cGMP信号转导系统在氯胺酮全麻机理中可能发挥重要作用。

To investigate the effects of ketamine on nitric oxide synathase(NOS)activity, nitrc oxide (NO) output and cyclic guanosine 3', 5'-monophosphate(cGMP)content in the rat brain. Method: Thirty two SD rats were divided randomly into control group and ketamine group. The aminals were administred intraperitoneally(ip)normal saline 10mg·kg^(-1) or ketamine 100mg·kg^(-1), respectively. NOS activity and NO output were assassed with spectrophotometric analysis, cGMP content was measured with radioimmunoassay, Result: Ketamine 100mg·kg^(-1) ip significantly inhibited NOS activity(P<0.05 or P<0.01), and reduced NO output and cGMP content (P<0.05 or P<0.01)in cerebellum, hippocampus and cerebral cortex as compared with those of control group. Conclusion: NO/cGMP signaling pathway may be associated with the molecular mechanism of ketamine in the general anesthesia.