HBV前C区1896位突变的检测方法及其评价

The Detection Method and its Evaluation of HBV Precore 1896nt Mutant

为建立一种简便可行的检测ＨＢＶ前Ｃ区１８９６位Ｇ→Ａ突变的方法。采用本室自行构建的ＨＢＶ前Ｃ区１８９６位Ｇ→Ａ突变株和野生株克隆作为标准株，对错配引物ＰＣＲ（ｍｐ－ＰＣＲ）结合限制性片段长度多态性分析（ＲＦＬＰ），作为检测ＨＢＶ前Ｃ区１８９６位Ｇ→Ａ突变株的方法进行了评价。结果显示，该方法操作较为简便，特异性和重复性均较满意。

For establishing a practical detection method of HBV precore 1896nt mutant, HBV precore 1896nt G>A mutant and wild clones constructed by our department as standard strains combined the mispairedPCR (mpPCR) with restricted fraction length polymorphism (RFLP) were used to detect HBV precore 1896nt mutant. The results show that the method is satisfied in specificity and repeatability with simpler procedure. It may be applied in clinical practice and research work.