野西瓜果实超临界CO_2流体萃取物的化学成分及药理活性

Chemical Constituents and Pharmacologic Activity of the Extracts from the Fruit of Capparis Spinosa by Supercritical Carbon Dioxide Fluid Extraction

采用气-质联用法对野西瓜(Capparis Spinosa)果实超临界CO_2流体萃取物的化学成分进行分析;应用小鼠扭体法和热板法研究其镇痛作用,应用二甲苯致小鼠耳肿胀法和角叉菜胶致大鼠足跖肿胀法研究其抗炎作用。结果检测出15个峰,占总峰面积的99.99%。其中含量较高的几种化合物有n-十六酸(8.34%),9,12-十八碳二烯酸乙酯(46.00%),9,12-十八碳二烯酸(31.01%),硬脂酸(1.74%),δ-维生素E(4.75%),β-维生素E(2.68%)等。研究表明野西瓜果实的超临界CO_2流体萃取物中化合物的类型主要为饱和脂肪酸酯、不饱和脂肪酸和甾醇类化合物等,萃取物具有明显的抗炎作用。

In order to study the chemical constituents of the extracts from the fruit of Capparis spinosa obtained by supercritical carbon dioxide fluid extraction （SFE-CO2） and its anti-inflammatory and analgesia effects. We analyzed the chemical constituents by GC-MS, and evaluated analgesia effect of the extracts by hot plate test and acetic acid-induced writhing test in mice, and observed the anti-inflammatory effect by means of ear oedema model induced by dimethylbenzene and rat voix pedis swell model induced by carrageenan. We identitied 15 chromatographical peaks which took about 99.99% of the total peak areas, including n-hexadecanoic acid （8.34%）, 9,12-octadecadienoic acid, ethyl ester （46.00%）, 9,12-octadecadienoic acid （Z,Z） （31.01%）, δ-tocopherol （4.75%） and β-tocopherol （2.68%）. The chemical components of the extracts were mainly saturated fatty acid esters, unsaturated fatty acids and sterinols. And the extracts had the potent anti-inflammatory effect.