廉价油脂青睐型脂肪酶产生菌株的筛选及其鉴定

Screening and Identification of Strains for Producing Cheap Oil Favorite Lipase

从本实验室分离到的96株产脂肪酶的菌株中筛选到9株具有转酯活性脂肪酶的菌株。通过气相色谱测定以三油酸甘油酯为底物的酶促转酯生成生物柴油的转酯效率,结果表明:F8和B10脂肪酶转酯活性相对较高,转酯率分别为48.64%和56.54%:分别以不同油脂为底物对这两株菌的脂肪酶进行底物偏好性试验,结果表明:B10对食用废弃油和麻疯树种子油的转酯能力最强,转化率分别为62.35%、58.60%;F8对麻疯树种子油的转酯率为26.82%,略高于其它油脂。对F8和B10进行biolog以及16S rDNA序列分析,鉴定结果显示F8和B10均为绿脓杆菌(Pseudomonas aeruginosa)。

Nine strains with transesterification activity were isolated from 96 lipase-producing strains ,which have been isolated by previous research in our laboratory. Analysis of the efficiency of transesterification in converting glycerol trioleate into biodiesel through gas chromatography showed that the strains F8 and B10 exhibited higher transesterifieation activity than other strains, the conversions of methyl ester were 48.64% and 56.54% respectively. Analysis of the substrate preference of lipases from these two strains with different oils as substrates showed that the strain B10 has the highest transesterification activity in converting food waste oils and Jatropha curcas L. seed oil into biodiesel, the efficiency of transesterification reached 62.35% and 58.60%, respectively; the strain F8 has the efficiency of transesterifieation of 26.82% in converting Jatropha curcas L. seed oil into biodiesel. The biolog and 16S rDNA sequence analysis showed that both of B10 and F8 were belonged to Pseudomonas aeruginosa.