流式细胞技术对尿中红细胞检测的临床价值

Clinical value of flow cytometry in detection of urinary RBC

目的评价流式细胞技术对尿红细胞检测的效能。方法随机留取尿液标本1318例,分别用UF-50尿沉渣分析仪和显微镜检测尿中红细胞。结果尿沉渣红细胞计数以显微镜检查为标准,UF-50尿沉渣分析仪测定尿红细胞阳性率(13.05%)高于镜检(9.18%),二者阳性符合率为70%。结论结晶、酵母菌、滴虫、脂肪滴、精子及大量细菌导致UF-50尿沉渣分析仪检测红细胞易受干扰,不能完全代替镜检,但可作为一种过筛和治疗监控的手段,有很高的临床应用价值。

Objective To study the efficacy of flow cytometry in measurement of urinary RBC. Methods A total of 1 318 urine samples were analyzed by using the UF-50 urinary sediment analysor and microscopic examination respectively. Results The positive rate of red blood cell（RBC） in urinary sediments tested with UF-50 analysor was 13.05%, higher than that by microscopic examination as the＇standard（9.18%）. Their positive coincidence was 700/00. Conclusion The detection of RBC with UF-50 analysor is influenced by crystals, yeast fungi, infusorium, fat drops, sperma and bacteria, which may lead to false results. Fully automated urinalysis analyzer can not replace the microscopic ex- amination, but it has very important clinical value as a sieving and monitoring means.