大鼠舌癌变过程中生存素、Bcl-2和p53蛋白的表达

Expression of survivin, Bcl-2 and p53 during 4-nitro-quinoline 1-oxide-induced rat tongue carcinogenesis

目的探讨生存素（survivin）蛋白在大鼠舌癌变过程中的表达及其与Bcl-2、p53蛋白表达的相关性。方法0．002％4-硝基喹啉-1-氧化物（4-nitro—quinoline 1-oxide，4NQO）饮水喂养SD大鼠9～36周建立大鼠舌癌变模型，用免疫组化二步法检测60例大鼠舌癌变过程中生存素、Bcl-2及p53蛋白的表达。结果36例正常组织中生存素蛋白表达仅1例，而11例异常增生组织中其表达为6例，11例口腔癌组织中其表达为10例。生存素蛋白在正常舌黏膜、异常增生黏膜及舌癌中的阳性表达差异有统计学意义（P〈0．001），异常增生及口腔癌组织中生存素蛋白表达显著高于正常黏膜中的表达（P〈0．01）。在17例生存素蛋白表达阳性的大鼠舌组织中，Bcl-2蛋白阳性表达12例，p53蛋白阳性表达8例；生存素蛋白表达与Bcl-2、p53蛋白表达呈正相关（P〈0．01）。结论生存素蛋白的表达增高和口腔鳞状上皮的异常增生、癌变有关，提示生存素可能参与了口腔癌的发生、发展；生存素、Bcl-2及p53蛋白的表达可能在口腔癌的发生、发展中起协调作用。

Objective To investigate the expression of survivin during oral carcinogenesis and its relationship with the expression of Bel-2 and p53. Methods Two-step immunohistoehemieal method was employed to detect the expression of survivin, Bcl-2 and p53 in 60 rat tongue carcinogenesis specimens induced by 4-nitro-quinoline 1-oxide （ 4NQO ）. Results Survivin was present in 1/36 eases of normal mueosa,6/ll eases of oral epithelial dysplasia and 10/11 cases of oral carcinoma. There were significant difference in the expression of survivin among normal mueosa, oral epithelial dysplasia and carcinoma （ P 〈 0. 001 ） . While in the 17 eases of positive expression of survivin,Bcl-2 was present in 12 eases and p53 was present in 8 eases. The expression of survivin in oral epithelial dysplasia and carcinoma were significantly higher than that in normal oral mueosa（P 〈 0. 01 ）. The expression of survivin was positively correlated to the expression of Bel-2 and p53 in rat tongue（ P 〈 0. 01 ）. Conclusions The expression of survivin may play an important role in oral carcinogenesis. Survivin, Bel-2 and p53 may be synergetic in the carcinogenesis of oral cancer.