汉族人内皮型一氧化氮合酶基因G894T多态性与冠心病关系的研究

Relationship between coronary heart disease and polymorphisms of endothelial nitric oxide synthase gene G894T in Han Nationality

目的:研究汉族人内皮型一氧化氮合酶(Endothelial nitric oxidesynthase,eNOS)基因G894T多态性与冠心病(Coronaryheart disease,CAD)的关系。方法:采用聚合酶链反应限制性片断长度多态性(Polymerase chain reaction-restricted fragments length polymorphism,PCR-RFLP)技术检测了103例CAD患者、104例对照组eNOS基因G894T多态性的基因型,分析了其对CAD的影响。结果:CAD组和对照组eNOS基因G894T多态性的等位基因频率有显著性差异(P=0.029),但是基因型分布在两组间的差异无统计学意义。Logistic回归分析确定高血压病、糖尿病及吸烟史为CAD发生的独立危险因素,T等位基因不是其发生的独立危险因素。结论:CAD组的eNOS基因G894T突变频率高于对照组,T等位基因可能与CAD的发生有一定的关联。

Objective：To explore the relationship between polymorphism of endothelial nitric oxide synthase （eNOS） gene G894T and coronary heart disease （CAD） in Han Nationlity. Methods： Polymerase chain reaction-restricted fragments length polymorphism （PCRRFLP） was used to determine the genotype of the eNOS gene G894T in 103 patients with CAD and 104 eontrols. Results：There was sig nificant difference in frequencies of allele in G894T polymorphism of eNOS gene between CAD and control groups （P=0.029）, but there was no significant difference in genotype distribution between two groups . Logistic regression analysis revealed that hypertension, diabete mellitus and smoking history were independent risk factors for the incidence of CAD,but T allele was not . Conclusion：The frequency of eNOS gene G894T mutation in the CAD group is higher than that in the control group and T allele may be related to with the development of CAD.