烟草悬凝物诱导永生化人支气管上皮细胞恶性转化过程中survivin基因及蛋白表达的研究

Study on survivin mRNA and protein of BEP-2D cells during its malignant transformation by cigarette smoke condensate

目的:探讨人支气管上皮细胞(BEP-2D)恶性转化过程中survivin基因及蛋白表达的变化。方法:选取正常BEP-2D细胞及经烟草悬凝物处理后的第15代(P-15)、25代(P-25)、38代(P-38)BEP-2D细胞共4组细胞株作为研究对象,采用半定量反转录PCR(Retro-translation PCR,RT-PCR)检测各组细胞株中survivin基因变化,免疫组织化检测各组中survivin蛋白的表达。结果:1.survivin mRNA在正常细胞BEP-2D中的表达强度为0.08,在转化的肺癌细胞P-15、P-25和P-38中分别为0.56、0.80和0.81。2.survivin蛋白在正常BEP-2D细胞株中表达阴性,在转化的3组细胞中表达阳性,且survivin蛋白在正常细胞与转化细胞株中的表达存在明显差异,其中在P-15具有明显差异(P<0.05)。结论:survivin基因及蛋白的表达水平可作为早期肺癌的一个检测指标。

Objective： To study the change of the survivin mRNA and protein of BEP-2D cells during its malignant transformation. Methods： Normal BEP-2D cell and BEP-2D cells treated by cigarette smoke condensate（ CSC ） for 15 weeks （P-15 ）, 25 weeks （P-25） and 38 weeks （P-38） were respectively chosen to study the survivin gene and protein by RT-PCR（retro-translation PCR, RT-PCR ）and immunohistochemical method. Results：The survivin mRNA was found in BEP-2D ceils of P-15,P-25 and P-38,respectively with 0.56,0.80,and 0.81 ,but not in the normal BEP-2D cell. The survivin protein was found in the normal BEP-2D cell,but not in BEP-2D cells of P-15,P-25 and P-38. The levels of survivin protein expressian were different between BEP-2D cells of P-15, P-25 and P-38 and normal BEP-2D cell ,with significant difference between P-15 BEP-2D cell and normal BEP-2D cell（P〈0.05 ）. Conclusion： The survivin mRNA and protein may be consider of a kind of biology-marker for the early diagnosis of lung cancer because the survivin mRNA and protein occur in the earlier period of lung cancer.