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强直性肌营养不良症患者肌肉组织中MyoD基因mRNA表达 被引量：2

MyoD mRNA expression in skeletal muscle of patients with myotonic dystrophy
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摘要 目的研究肌分化因子MyoD基因mRNA在强直性肌营养不良症（DM）患者肌肉组织中的表达情况。方法采用半定量RT-PCR方法检测4例DM肌肉组织和配对非肌肉病对照者肌肉组织MyoD基因mRNA表达。结果两组肌肉组织中均MyoD基因mRNA表达；DM肌肉组织中MyoDmRNA的表达指数为0．267±0．114，明显低于对照组肌肉组织（0．788±0．136），二者差异有统计学意义（P=0．001）。结论DM肌肉组织中存在MyoD基因mRNA表达显著下调。 Objective To explore the mRNA expression of MyoD gene in the skeletal muscles of myotonic dystrophy （MD） patients. Methods Muscle biopsy specimens were obtained from the biceps muscles of arm of 4 MD patients and 4 healthy controls. Semi-quantitative reverse transcription polymerase chain reaction was performed to evaluate the mRNA expression of MyoD in the specimens. Results The mRNA expression index of MyoD of the MD patients was （0. 267 ± 0. 114 ）, significantly lower than that of the healthy controls [ （ 0. 788 ± 0. 136） ,P = 0. 0011. Conclusion Down-regulated mRNA expression of MyoD gene is involved in the mechanism of DM.
作者 赵晓萍 蒲传强 段枫 刘洁晓 毛燕玲 罗萍
机构地区 解放军总医院神经内科
出处 《中华医学杂志》 CAS CSCD 北大核心 2009年第7期466-468,共3页 National Medical Journal of China
关键词 肌强直 营养不良性 转化生长因子Β 基因表达 Myotonie dystrophy Transforming growth factor beta Gene expression
分类号 R686 [医药卫生—骨科学]
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参考文献9

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二级参考文献11

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中华医学杂志
2009年 第7期

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