牛磺酸对环磷酰胺增效减毒作用的研究

Synergism and attenuation effects of taurine on cyclophosphamide

背景与目的:环磷酰胺(cyclophosphamide,CTX)是临床常用的抗肿瘤药物,但是不良反应较大,因此,寻找实现增效减毒的辅助药物有重要意义。本研究探讨两种给药途径下牛磺酸(taurine,Tau)对CTX的增效减毒作用。方法:以S180荷瘤小鼠为模型,Tau采用灌胃和静脉注射两种给药途径与CTX联合用药。检测各组小鼠的抑瘤率、骨髓有核细胞数、外周血白细胞数、脾指数、胸腺指数、脾淋巴细胞增殖活性和腹腔巨噬细胞吞噬能力等指标,分析Tau对CTX的增效减毒作用。结果:Tau(40mg·kg-1、80mg·kg-1、160mg·kg-1)静脉注射或Tau(160mg·kg-1、320mg·kg-1、640mg·kg-1)灌胃联合应用CTX(20mg·kg-1)腹腔注射对S180肉瘤的抑瘤率均比单一使用CTX高,CTX组抑瘤率为55.8%,而Tau(静脉注射)+CTX低、中、高各剂量组的抑瘤率分别是66.4%、74.5%和84.6%,Tau(灌胃)+CTX低、中、高各剂量组的抑瘤率分别是60.1%、69.7%和81.2%;与CTX组比较,Tau(静脉注射)+CTX各剂量组和Tau(灌胃)+CTX各剂量组外周血白细胞数、骨髓有核细胞数、胸腺指数、脾指数、脾淋巴细胞增殖活性和腹腔巨噬细胞吞噬能力均增高。结论:两种给药途径下Tau对CTX均具有增效减毒作用。

Background and Objective： Cyclophosphamide （CTX） is a commonly used clinical antitumor drug with severe side effects. Therefore, it is important to find ancillary drugs which have synergism and attenuation effects on CTX. This study was to investigate the synergism and attenuation effects of taurine （Tau） on CTX via different administration methods. Methods： S180-bearing mice were given Tau combined with CTX via either intravenous injection or intragastric administration. The tumor inhibition rate, the count of bone marrow nucleate cells and white blood cells, the spleen index, the thymus index, lymphocyte proliferation and the phagocytic activity of peritoneal macrophage were calculated. Results： The tumor inhibition rates of intravenous administration of 40 mg·kg^-1, 80 mg·kg^-1, 160 mg·kg^-1 Tau combined with CTX （20 mg·kg^-1） were 66.4%, 74.5% and 84.6%, while those of intragastric administration of 160 mg·kg^-1, 320 mg·kg^-1, 640 mg·kg^-1 Tau with CTX （20 mg·kg^-1） were 60.1%, 69.7% and 81.2%, all of which were higher than that of CTX administration （20 mg·g^-1） alone （55.8%）. Compared to the CTX group, the count of bone marrow nucleate cells and the white blood cells, the spleen index, the thymus index, lymphocyte proliferation and the phagocytic activity of peritoneal macrophage were elevated in all Tau and CTX combination groups. Conclusion： Tau has synergism and attenuation effects on CTX via both intravenous and intragastric administration.